Application | Comment | Organism |
---|---|---|
industry | the purified enzyme reveales its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids | uncultured Burkholderiaceae bacterium |
synthesis | the purified enzyme reveales its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids | uncultured Burkholderiaceae bacterium |
Cloned (Comment) | Organism |
---|---|
expression into Escherichia coli BL21 (DE3) | uncultured Burkholderiaceae bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | weak inhibition | uncultured Burkholderiaceae bacterium | |
CoCl2 | weak inhibition | uncultured Burkholderiaceae bacterium | |
Cu2+ | strong inhibition | uncultured Burkholderiaceae bacterium | |
Hg2+ | strong inhibition | uncultured Burkholderiaceae bacterium | |
additional information | the enzyme retains near maximal activity even in the presence of high concentrations of ZnCl2, MgSO4 and MnSO4 | uncultured Burkholderiaceae bacterium | |
NaCl | the enzyme loses more than 75% of its activity at NaCl concentrations higher than 2.0 mol/l | uncultured Burkholderiaceae bacterium | |
NiSO4 | weak inhibition | uncultured Burkholderiaceae bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
59.4 | - |
Succinonitrile | pH 7.2, 40°C | uncultured Burkholderiaceae bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38830 | - |
calculated from sequence | uncultured Burkholderiaceae bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | G5DDB2 | - |
- |
Cereibacter sphaeroides LHS-305 | G5DDB2 | - |
- |
uncultured Burkholderiaceae bacterium | A0A140D8I8 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged enzyme | uncultured Burkholderiaceae bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaronitrile + H2O | activity towards glutaronitrile is 8.5% of that towards succinonitrile | uncultured Burkholderiaceae bacterium | glutaric acid + NH3 | - |
? | |
additional information | no activity with mandelonitrile, arylacetonitrile and benzonitrile. No activity with acetamide and benzamide | uncultured Burkholderiaceae bacterium | ? | - |
- |
|
succinonitrile + H2O | - |
uncultured Burkholderiaceae bacterium | succinic acid + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NitraS-ATII | - |
Cereibacter sphaeroides |
NitraS-ATII | - |
uncultured Burkholderiaceae bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
uncultured Burkholderiaceae bacterium |
50 | - |
- |
Cereibacter sphaeroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | 30°C: about 65% of maximal activity, 45°C: about 25% of maximal activity | uncultured Burkholderiaceae bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
1 min, about 10% loss of activity | uncultured Burkholderiaceae bacterium |
50 | - |
1 min, about 25% loss of activity. 60 min, about 85% loss of activity | uncultured Burkholderiaceae bacterium |
55 | - |
1 min, about 25% loss of activity | uncultured Burkholderiaceae bacterium |
60 | - |
1 min, about 40% loss of activity | uncultured Burkholderiaceae bacterium |
65 | - |
1 min, about 40% loss of activity | uncultured Burkholderiaceae bacterium |
70 | - |
1 min, about 40% loss of activity | uncultured Burkholderiaceae bacterium |
75 | - |
1 min, about 70% loss of activity | uncultured Burkholderiaceae bacterium |
80 | - |
1 min, about 95% loss of activity | uncultured Burkholderiaceae bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.472 | - |
Succinonitrile | pH 7.2, 40°C | uncultured Burkholderiaceae bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
uncultured Burkholderiaceae bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 40% of maximal activity, pH 9.0: about 55% of maximal activity | uncultured Burkholderiaceae bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
Succinonitrile | pH 7.2, 40°C | uncultured Burkholderiaceae bacterium |