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Literature summary for 3.5.5.1 extracted from

  • Sonbol, S.A.; Ferreira, A.J.; Siam, R.
    Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance (2016), BMC Biotechnol., 16, 14 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
industry the purified enzyme reveales its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids uncultured Burkholderiaceae bacterium
synthesis the purified enzyme reveales its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids uncultured Burkholderiaceae bacterium

Cloned(Commentary)

Cloned (Comment) Organism
expression into Escherichia coli BL21 (DE3) uncultured Burkholderiaceae bacterium

Inhibitors

Inhibitors Comment Organism Structure
CdCl2 weak inhibition uncultured Burkholderiaceae bacterium
CoCl2 weak inhibition uncultured Burkholderiaceae bacterium
Cu2+ strong inhibition uncultured Burkholderiaceae bacterium
Hg2+ strong inhibition uncultured Burkholderiaceae bacterium
additional information the enzyme retains near maximal activity even in the presence of high concentrations of ZnCl2, MgSO4 and MnSO4 uncultured Burkholderiaceae bacterium
NaCl the enzyme loses more than 75% of its activity at NaCl concentrations higher than 2.0 mol/l uncultured Burkholderiaceae bacterium
NiSO4 weak inhibition uncultured Burkholderiaceae bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
59.4
-
Succinonitrile pH 7.2, 40°C uncultured Burkholderiaceae bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38830
-
calculated from sequence uncultured Burkholderiaceae bacterium

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides G5DDB2
-
-
Cereibacter sphaeroides LHS-305 G5DDB2
-
-
uncultured Burkholderiaceae bacterium A0A140D8I8
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged enzyme uncultured Burkholderiaceae bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaronitrile + H2O activity towards glutaronitrile is 8.5% of that towards succinonitrile uncultured Burkholderiaceae bacterium glutaric acid + NH3
-
?
additional information no activity with mandelonitrile, arylacetonitrile and benzonitrile. No activity with acetamide and benzamide uncultured Burkholderiaceae bacterium ?
-
-
succinonitrile + H2O
-
uncultured Burkholderiaceae bacterium succinic acid + NH3
-
?

Synonyms

Synonyms Comment Organism
NitraS-ATII
-
Cereibacter sphaeroides
NitraS-ATII
-
uncultured Burkholderiaceae bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
uncultured Burkholderiaceae bacterium
50
-
-
Cereibacter sphaeroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 65% of maximal activity, 45°C: about 25% of maximal activity uncultured Burkholderiaceae bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
1 min, about 10% loss of activity uncultured Burkholderiaceae bacterium
50
-
1 min, about 25% loss of activity. 60 min, about 85% loss of activity uncultured Burkholderiaceae bacterium
55
-
1 min, about 25% loss of activity uncultured Burkholderiaceae bacterium
60
-
1 min, about 40% loss of activity uncultured Burkholderiaceae bacterium
65
-
1 min, about 40% loss of activity uncultured Burkholderiaceae bacterium
70
-
1 min, about 40% loss of activity uncultured Burkholderiaceae bacterium
75
-
1 min, about 70% loss of activity uncultured Burkholderiaceae bacterium
80
-
1 min, about 95% loss of activity uncultured Burkholderiaceae bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.472
-
Succinonitrile pH 7.2, 40°C uncultured Burkholderiaceae bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
uncultured Burkholderiaceae bacterium

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 55% of maximal activity uncultured Burkholderiaceae bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0079
-
Succinonitrile pH 7.2, 40°C uncultured Burkholderiaceae bacterium