Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.1 extracted from

  • Xue, Y.P.; Jiao, B.; Hua, D.E.; Cheng, F.; Liu, Z.Q.; Zheng, Y.G.
    Improving catalytic performance of an arylacetonitrilase by semirational engineering (2017), Bioprocess Biosyst. Eng., 40, 1565-1572 .
    View publication on PubMed
Search for more literature for 3.5.5.1

Protein Variants

Protein Variants Comment Organism
S190G mutant enzyme exhibits 3fold higher specific activity toward mandelonitrile compared with that of wild-type uncultured organism

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 mandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
1.98
-
 mandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.89
-
 2-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.98
-
 2-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
5.47
-
 3-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
5.67
-
 3-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
6.08
-
 4-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
6.15
-
 4-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism

Organism

Organism UniProt Comment Textmining
uncultured organism Q6RWE0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloromandelonitrile + H2O
-
 uncultured organism 2-chloromandelic acid + NH3
-
 ?
3-chloromandelonitrile + H2O
-
 uncultured organism 3-chloromandelic acid + NH3
-
 ?
4-chloromandelonitrile + H2O
-
 uncultured organism 3-chloromandelic acid + NH3
-
 ?
mandelonitrile + H2O
-
 uncultured organism mandelic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NitA
-
 uncultured organism

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 2-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
1.82
-
 2-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
1.98
-
 3-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
2.2
-
 4-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.07
-
 4-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.41
-
 3-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
4.6
-
 mandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
13.23
-
 mandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.33
-
 2-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
0.35
-
 3-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
0.36
-
 4-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
0.47
-
 2-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
0.5
-
 4-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
0.62
-
 3-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
2.33
-
 mandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
17.38
-
 mandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism