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Literature summary for 3.5.5.1 extracted from

  • Martinkova, L.; Vesela, A.B.; Rinagelova, A.; Chmatal, M.
    Cyanide hydratases and cyanide dihydratases emerging tools in the biodegradation and biodetection of cyanide (2015), Appl. Microbiol. Biotechnol., 99, 8875-8882 .
    View publication on PubMed

Application

Application Comment Organism
analysis tools in the biodegradation and biodetection of cyanide Bacillus pumilus

Protein Variants

Protein Variants Comment Organism
E327G/Q86R/E96G/D254E/E35K/Q322R/E327G/Q86R/E6G/D254E/E327G mutamt enzyme shows activity at pH 10. The wild-type enzyme exhibits the optimum activity at pH 8 and is not able to hydrolyze HCN at pH higher than 9. However, cyanide wastes should be maintained at alkaline pH to prevent HCN release. Therefore, the CynD from Bacillus pumilus was engineered for an increased activity under these conditions (pH 9-10), and several mutants were shown to meet this requirement Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus B3GNT7
-
-

Synonyms

Synonyms Comment Organism
CynD
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Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
wild-type enzyme Bacillus pumilus