Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
K96R | retains the ability to hydrolyse amides but hydrolyses nitriles more efficiently than the wild-type enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethanesulfonyl fluoride | inhibits amide conversion more than 94% and completely suppresses nitrile hydrolysis | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000005 | - |
trichloroacetonitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.000000009 | - |
phenylglycinenitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.0000000097 | - |
Cinnamonitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.000000024 | - |
phenylglycinenitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.0000000495 | - |
Malononitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.000000103 | - |
3-anilinoproprionitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.00000013 | - |
trichloroacetonitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.000000157 | - |
3-anilinoproprionitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.000000609 | - |
Malononitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.000005 | - |
Benzonitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.000006 | - |
Benzonitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P95896 | - |
- |
Saccharolobus solfataricus P2 | P95896 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0006 | - |
pH 7.4, 70°C, substrate: benzonitrile | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-anilinoproprionitrile + 2 H2O | - |
Saccharolobus solfataricus | 3-anilinopropionate + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Saccharolobus solfataricus | benzoate + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Saccharolobus solfataricus P2 | benzoate + NH3 | - |
? | |
cinnamonitrile + 2 H2O | cinnamonitrile is a substrate for mutant enzyme K96R, no activity with wild-type enzyme | Saccharolobus solfataricus | cinnamic acid + NH3 | - |
? | |
malononitrile + 2 H2O | - |
Saccharolobus solfataricus | malonate + NH3 | - |
? | |
phenylglycinenitrile + 2 H2O | - |
Saccharolobus solfataricus | phenylglycine + NH3 | - |
? | |
trichloroacetonitrile + 2 H2O | - |
Saccharolobus solfataricus | trichloroacetate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SsAH | - |
Saccharolobus solfataricus |
SSO2122 | ordered locus name | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
enzyme remains active for about 6 days | Saccharolobus solfataricus |
95 | - |
half-life: 5 h | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
trichloroacetonitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.016 | - |
Cinnamonitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.016 | - |
phenylglycinenitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.04 | - |
phenylglycinenitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.09 | - |
Benzonitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.14 | - |
Malononitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.18 | - |
3-anilinoproprionitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.225 | - |
trichloroacetonitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus | |
0.28 | - |
3-anilinoproprionitrile | pH 7.4, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
1.05 | - |
Malononitrile | pH 7.4, 70°C, mutant enzyme K96R | Saccharolobus solfataricus |