Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful fpr synthesis of iminodiacetic acid, which is widely used as an intermediate in the manufacture of chelating agents, glyphosate herbicides and surfactants | Acidovorax facilis |
Cloned (Comment) | Organism |
---|---|
functional expression in Escherichia coli strain BL21 (DE3) | Acidovorax facilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization and stabilization of Escherichia coli cells in 8.0% polyvinyl alcohol and 1.0% sodium alginate copolymer recombinantly expressing the Acidovorax facilis nitrilase for stable production of iminoacetic acid, method optimization, overview. Maximum relative nitrilase activity with 1.0% CaCl2, and 5.0% wet cells, with 1.0% iminodiacetonitrile in distilled water at 40°C. Substrate specificity of immobilized and free cells, overview. Ca2+ can stabilize the cells, but is toxic at concentrations above 1% leading to a sharp decrease in nitrilase activity of immobilized cells. Immobilized and free cells display different sensitivity towards temperatures ranging from 25°C to 70°C, with optimal tempartures of 40°C and 45°C, respectively. Comparison of operational and storage stability of free and immobilized cells, overview | Acidovorax facilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at concentrations of CaCl2 of 1.0-5.0%, the activity of nitrilase decreases sharply, higher concentration of CaCl 2 seem to have a poisoning effect on the cells | Acidovorax facilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, about, sequence calculation and SDS-PAGE | Acidovorax facilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax facilis | Q1W2L4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44 | - |
enzyme activity in immobilized cells, pH and temperature not specified in the publication | Acidovorax facilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-dimethylcyclopropyl cyanide + 2 H2O | - |
Acidovorax facilis | 2,2-dimethylcyclopropyl carboxylate + NH3 | - |
? | |
2-amino-2,3-dimethylbutanenitrile + 2 H2O | - |
Acidovorax facilis | ? + NH3 | - |
? | |
2-aminobutyronitrile + 2 H2O | high activity | Acidovorax facilis | 2-aminobutyric acid + NH3 | - |
? | |
3-hydroxy-glutaronitrile + 2 H2O | - |
Acidovorax facilis | 3-hydroxy-glutaric acid + NH3 | - |
? | |
4-aminobenzonitrile + 2 H2O | - |
Acidovorax facilis | 4-aminobenzoic acid + NH3 | - |
? | |
4-methoxybenzyl cyanide + 2 H2O | very low activity | Acidovorax facilis | 4-methoxyphenylacetic acid + NH3 | - |
? | |
acetonitrile + 2 H2O | - |
Acidovorax facilis | acetic acid + NH3 | - |
? | |
acrylonitrile + 2 H2O | very low activity | Acidovorax facilis | acrylic acid + NH3 | - |
? | |
adiponitrile + 2 H2O | high activity | Acidovorax facilis | adipic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Acidovorax facilis | benzoic acid + NH3 | - |
? | |
glycolonitrile + 2 H2O | high activity | Acidovorax facilis | glycolic acid + NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | high activity | Acidovorax facilis | iminodiacetic acid + 2 NH3 | - |
? | |
additional information | substrate specificity of immobilized and free Escherichia coli cells recombinantly expressing the Acidovorax facilis nitrilase, overview. No activity with mandelonitrile, o-methoxyphenylacetonitrile, m-methoxyphenylacetonitrile, p-methoxyphenylacetonitrile, and phenylacetonitrile | Acidovorax facilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, about, sequence calculation and SDS-PAGE | Acidovorax facilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
in vivo assay, recombinant cells | Acidovorax facilis |