Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | slightly activating at 1 mM, not at 5 mM | Alcaligenes sp. |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pSH | Alcaligenes sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Alcaligenes sp. | |
Ca2+ | - |
Alcaligenes sp. | |
Co2+ | - |
Alcaligenes sp. | |
Cu2+ | - |
Alcaligenes sp. | |
EDTA | - |
Alcaligenes sp. | |
Fe2+ | - |
Alcaligenes sp. | |
Fe3+ | - |
Alcaligenes sp. | |
Hg2+ | - |
Alcaligenes sp. | |
Li+ | - |
Alcaligenes sp. | |
Mg2+ | - |
Alcaligenes sp. | |
Ni2+ | - |
Alcaligenes sp. | |
Pb2+ | - |
Alcaligenes sp. | |
Zn2+ | - |
Alcaligenes sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.8 | - |
(R,S)-2-hydroxy-2-phenylacetonitrile | pH 8.0, 40°C, recombinant enzyme | Alcaligenes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slightly activating at 1 mM, slightly inhibitory at 5 mM | Alcaligenes sp. | |
Li+ | activating at 1 mM to 133%, slightly inhibitory at 5 mM | Alcaligenes sp. | |
Mn2+ | slightly activating at 1 mM, not at 5 mM | Alcaligenes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
376000 | - |
recombinant enzyme, gel filtration | Alcaligenes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | C5IIS9 | - |
- |
Alcaligenes sp. ECU0401 | C5IIS9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)/pSH by nickel affinity chromatography | Alcaligenes sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19 | - |
substrate mandelonitrile, purified recombinant enzyme, pH 8.0, 40°C | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-hydroxy-2-phenylacetonitrile + 2 H2O | i.e. (R,S)-mandelonitrile, preferred substrate, highly enantioselective reaction | Alcaligenes sp. | (R)-2-hydroxy-2-phenylacetic acid + NH3 | - |
? | |
(R,S)-2-hydroxy-2-phenylacetonitrile + 2 H2O | i.e. (R,S)-mandelonitrile, preferred substrate, highly enantioselective reaction | Alcaligenes sp. ECU0401 | (R)-2-hydroxy-2-phenylacetic acid + NH3 | - |
? | |
2-chloromandelonitrile + 2 H2O | - |
Alcaligenes sp. | 2-chloromandelic acid + NH3 | - |
? | |
2-chloromandelonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | 2-chloromandelic acid + NH3 | - |
? | |
2-phenylglycinonitrile + 2 H2O | - |
Alcaligenes sp. | amino(phenyl)acetic acid + NH3 | - |
? | |
acetonitrile + 2 H2O | - |
Alcaligenes sp. | acetic acid + NH3 | - |
? | |
acetonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | acetic acid + NH3 | - |
? | |
benzyl cyanide + 2 H2O | - |
Alcaligenes sp. | phenylacetic acid + NH3 | - |
? | |
glycolonitrile + 2 H2O | - |
Alcaligenes sp. | glycolic acid + NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | - |
Alcaligenes sp. | iminodiacetic acid + 2 NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | iminodiacetic acid + 2 NH3 | - |
? | |
additional information | substrate specificity, overview. No activity with diaminomaleonitrile, 2,3-dicyanopyrazine, 4-hydroxybenzonitrile, 2-cyano-3-phenylpropionitrile, 4-aminophthalonitrile, and 4-nitrophthalonitrile | Alcaligenes sp. | ? | - |
? | |
additional information | substrate specificity, overview. No activity with diaminomaleonitrile, 2,3-dicyanopyrazine, 4-hydroxybenzonitrile, 2-cyano-3-phenylpropionitrile, 4-aminophthalonitrile, and 4-nitrophthalonitrile | Alcaligenes sp. ECU0401 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | - |
Alcaligenes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Alcaligenes sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
purified recombinant enzyme, half-life is 4 h | Alcaligenes sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alcaligenes sp. | two-dimensional electrophoresis | - |
5.28 |