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Literature summary for 3.5.5.1 extracted from

  • Vejvoda, V.; Kubac, D.; Davidova, A.; Kaplan, O.; Sulc, M.; Sveda, O.; Chaloupkova, R.; Martinkova, L.
    Purification and characterization of nitrilase from Fusarium solani IMI196840 (2010), Process Biochem., 45, 1115-1120.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-Cyanopyridine nitrilase inducer Fusarium solani

Inhibitors

Inhibitors Comment Organism Structure
(CH3CO2)2Pb 13.9% residual activity at 1 mM Fusarium solani
2-mercaptoethanol 90.2% residual activity at 5 mM Fusarium solani
Ag+ 1.7% residual activity at 1 mM Fusarium solani
Al3+ 0.5% residual activity at 5 mM Fusarium solani
Co2+ 75.8% residual activity at 1 mM Fusarium solani
Cr3+ 27.8% residual activity at 1 mM Fusarium solani
Cu2+ 88.3% residual activity at 1 mM Fusarium solani
dithiothreitol 79% residual activity at 1 mM Fusarium solani
EDTA 84.1% residual activity at 5 mM Fusarium solani
Fe3+ 43.7% residual activity at 5 mM Fusarium solani
Hg2+ 0.1% residual activity at 1 mM Fusarium solani
Mg2+ 85.3% residual activity at 5 mM Fusarium solani
Mn2+ 79.3% residual activity at 1 mM Fusarium solani
Ni2+ 62% residual activity at 1 mM Fusarium solani
Zn2+ 8.2% residual activity at 1 mM Fusarium solani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Fusarium solani
550000
-
gel filtration Fusarium solani
580000
-
gel filtration Fusarium solani

Organism

Organism UniProt Comment Textmining
Fusarium solani
-
-
-
Fusarium solani IMI196840
-
-
-
Fusarium solani O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, and Q-Sepharose column chromatography Fusarium solani

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
after 20.3fold purification, the specific activity is 144 units/mg, while the crude enzyme exhibits a specific activity of 7.1 units/mg at pH 8.0 and 45°C Fusarium solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyanopyridine + 2 H2O 7% activity compared to benzonitrile Fusarium solani 2-pyridinecarboxylic acid + NH3
-
?
3-aminobenzonitrile + H2O 2% activity compared to benzonitrile Fusarium solani 3-aminobenzoic acid + NH3
-
?
3-chlorobenzonitrile + H2O 11% activity compared to benzonitrile Fusarium solani 3-chlorobenzoic acid + NH3
-
?
3-chlorobenzonitrile + H2O 11% activity compared to benzonitrile Fusarium solani IMI196840 3-chlorobenzoic acid + NH3
-
?
3-chlorobenzonitrile + H2O 11% activity compared to benzonitrile Fusarium solani O1 3-chlorobenzoic acid + NH3
-
?
3-cyanopyridine + 2 H2O 41% activity compared to benzonitrile Fusarium solani 3-pyridinecarboxylic acid + NH3
-
?
3-hydroxybenzonitrile + H2O 1% activity compared to benzonitrile Fusarium solani 3-hydroxybenzoic acid + NH3
-
?
3-tolunitrile + H2O 9% activity compared to benzonitrile Fusarium solani 3-methylbenzoate + NH3
-
?
4-chlorobenzonitrile + H2O 19% activity compared to benzonitrile Fusarium solani 4-chlorobenzoic acid + NH3
-
?
4-chlorobenzonitrile + H2O 19% activity compared to benzonitrile Fusarium solani IMI196840 4-chlorobenzoic acid + NH3
-
?
4-chlorobenzonitrile + H2O 19% activity compared to benzonitrile Fusarium solani O1 4-chlorobenzoic acid + NH3
-
?
4-cyanopyridine + 2 H2O best substrate showing 217% activity compared to benzonitrile Fusarium solani 4-pyridinecarboxylic acid + NH3
-
?
4-cyanopyridine + 2 H2O best substrate showing 217% activity compared to benzonitrile Fusarium solani IMI196840 4-pyridinecarboxylic acid + NH3
-
?
4-cyanopyridine + 2 H2O best substrate showing 217% activity compared to benzonitrile Fusarium solani O1 4-pyridinecarboxylic acid + NH3
-
?
4-tolunitrile + H2O 5% activity compared to benzonitrile Fusarium solani 4-methylbenzoate + NH3
-
?
benzonitrile + 2 H2O 100% activity Fusarium solani benzoic acid + NH3
-
?
benzonitrile + 2 H2O 100% activity Fusarium solani IMI196840 benzoic acid + NH3
-
?
benzonitrile + 2 H2O 100% activity Fusarium solani O1 benzoic acid + NH3
-
?
additional information the enzyme is highly chemoselective, producing less than 2% amides as by-products Fusarium solani ?
-
?
additional information the enzyme is highly chemoselective, producing less than 2% amides as by-products Fusarium solani IMI196840 ?
-
?
additional information the enzyme is highly chemoselective, producing less than 2% amides as by-products Fusarium solani O1 ?
-
?

Subunits

Subunits Comment Organism
homooligomer x * 40000, SDS-PAGE Fusarium solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Fusarium solani
45
-
-
Fusarium solani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50
-
Fusarium solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46.8
-
the melting temperature of the enzyme is 46.8°C Fusarium solani
49.3
-
the melting temperature of the enzyme is 49.3°C Fusarium solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Fusarium solani
8
-
-
Fusarium solani

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5
-
Fusarium solani
7 9
-
Fusarium solani