Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-Cyanopyridine | nitrilase inducer | Fusarium solani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(CH3CO2)2Pb | 13.9% residual activity at 1 mM | Fusarium solani | |
2-mercaptoethanol | 90.2% residual activity at 5 mM | Fusarium solani | |
Ag+ | 1.7% residual activity at 1 mM | Fusarium solani | |
Al3+ | 0.5% residual activity at 5 mM | Fusarium solani | |
Co2+ | 75.8% residual activity at 1 mM | Fusarium solani | |
Cr3+ | 27.8% residual activity at 1 mM | Fusarium solani | |
Cu2+ | 88.3% residual activity at 1 mM | Fusarium solani | |
dithiothreitol | 79% residual activity at 1 mM | Fusarium solani | |
EDTA | 84.1% residual activity at 5 mM | Fusarium solani | |
Fe3+ | 43.7% residual activity at 5 mM | Fusarium solani | |
Hg2+ | 0.1% residual activity at 1 mM | Fusarium solani | |
Mg2+ | 85.3% residual activity at 5 mM | Fusarium solani | |
Mn2+ | 79.3% residual activity at 1 mM | Fusarium solani | |
Ni2+ | 62% residual activity at 1 mM | Fusarium solani | |
Zn2+ | 8.2% residual activity at 1 mM | Fusarium solani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Fusarium solani |
550000 | - |
gel filtration | Fusarium solani |
580000 | - |
gel filtration | Fusarium solani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium solani | - |
- |
- |
Fusarium solani IMI196840 | - |
- |
- |
Fusarium solani O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, and Q-Sepharose column chromatography | Fusarium solani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
after 20.3fold purification, the specific activity is 144 units/mg, while the crude enzyme exhibits a specific activity of 7.1 units/mg at pH 8.0 and 45°C | Fusarium solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-cyanopyridine + 2 H2O | 7% activity compared to benzonitrile | Fusarium solani | 2-pyridinecarboxylic acid + NH3 | - |
? | |
3-aminobenzonitrile + H2O | 2% activity compared to benzonitrile | Fusarium solani | 3-aminobenzoic acid + NH3 | - |
? | |
3-chlorobenzonitrile + H2O | 11% activity compared to benzonitrile | Fusarium solani | 3-chlorobenzoic acid + NH3 | - |
? | |
3-chlorobenzonitrile + H2O | 11% activity compared to benzonitrile | Fusarium solani IMI196840 | 3-chlorobenzoic acid + NH3 | - |
? | |
3-chlorobenzonitrile + H2O | 11% activity compared to benzonitrile | Fusarium solani O1 | 3-chlorobenzoic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | 41% activity compared to benzonitrile | Fusarium solani | 3-pyridinecarboxylic acid + NH3 | - |
? | |
3-hydroxybenzonitrile + H2O | 1% activity compared to benzonitrile | Fusarium solani | 3-hydroxybenzoic acid + NH3 | - |
? | |
3-tolunitrile + H2O | 9% activity compared to benzonitrile | Fusarium solani | 3-methylbenzoate + NH3 | - |
? | |
4-chlorobenzonitrile + H2O | 19% activity compared to benzonitrile | Fusarium solani | 4-chlorobenzoic acid + NH3 | - |
? | |
4-chlorobenzonitrile + H2O | 19% activity compared to benzonitrile | Fusarium solani IMI196840 | 4-chlorobenzoic acid + NH3 | - |
? | |
4-chlorobenzonitrile + H2O | 19% activity compared to benzonitrile | Fusarium solani O1 | 4-chlorobenzoic acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | best substrate showing 217% activity compared to benzonitrile | Fusarium solani | 4-pyridinecarboxylic acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | best substrate showing 217% activity compared to benzonitrile | Fusarium solani IMI196840 | 4-pyridinecarboxylic acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | best substrate showing 217% activity compared to benzonitrile | Fusarium solani O1 | 4-pyridinecarboxylic acid + NH3 | - |
? | |
4-tolunitrile + H2O | 5% activity compared to benzonitrile | Fusarium solani | 4-methylbenzoate + NH3 | - |
? | |
benzonitrile + 2 H2O | 100% activity | Fusarium solani | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 100% activity | Fusarium solani IMI196840 | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 100% activity | Fusarium solani O1 | benzoic acid + NH3 | - |
? | |
additional information | the enzyme is highly chemoselective, producing less than 2% amides as by-products | Fusarium solani | ? | - |
? | |
additional information | the enzyme is highly chemoselective, producing less than 2% amides as by-products | Fusarium solani IMI196840 | ? | - |
? | |
additional information | the enzyme is highly chemoselective, producing less than 2% amides as by-products | Fusarium solani O1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooligomer | x * 40000, SDS-PAGE | Fusarium solani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Fusarium solani |
45 | - |
- |
Fusarium solani |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | - |
Fusarium solani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46.8 | - |
the melting temperature of the enzyme is 46.8°C | Fusarium solani |
49.3 | - |
the melting temperature of the enzyme is 49.3°C | Fusarium solani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Fusarium solani |
8 | - |
- |
Fusarium solani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | - |
Fusarium solani |
7 | 9 | - |
Fusarium solani |