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Literature summary for 3.5.5.1 extracted from
- Microbial nitrilases: Versatile, spiral forming, industrial enzymes (2009), J. Appl. Microbiol., 106, 703-727.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Benzonitrile | - |
Rhodococcus sp. |  |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Rhodococcus rhodochrous |
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Arthrobacter sp. |
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Rhodococcus sp. |
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Fusarium solani |
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Aspergillus niger |
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Aeribacillus pallidus |
| industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Fusarium oxysporum f. sp. melonis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
phenylpropionitrile | pH and temperature not specified in the publication | Brassica napus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
- |
Arthrobacter sp. |
| 34500 | - |
10 * 34500 | Bradyrhizobium japonicum |
| 37000 | - |
14 * 37000 | Fusarium oxysporum f. sp. melonis |
| 38000 | - |
10 * 38000 or 11 * 38000 | Brassica napus |
| 38000 | - |
14 * 38000 | Arabidopsis thaliana |
| 38500 | - |
10 * 38500 or 12 * 38500 | Aspergillus niger |
| 40000 | - |
14 * 40000 | Rhodococcus rhodochrous |
| 40000 | - |
14 * 40000 | Fusarium solani |
| 41000 | - |
14 * 41000 | Aeribacillus pallidus |
| 45000 | - |
- |
Rhodococcus rhodochrous |
| 45000 | - |
12 * 45000 | Rhodococcus sp. |
| 45800 | - |
12 * 45800 | Rhodococcus sp. |
| 76000 | - |
8 * 76000 | Fusarium solani |
| 340000 | - |
- |
Bradyrhizobium japonicum |
| 420000 | - |
- |
Brassica napus |
| 450000 | - |
- |
Arabidopsis thaliana |
| 550000 | - |
- |
Fusarium oxysporum f. sp. melonis |
| 560000 | - |
- |
Rhodococcus rhodochrous |
| 560000 | - |
- |
Rhodococcus sp. |
| 580000 | - |
- |
Fusarium solani |
| 600000 | - |
- |
Aeribacillus pallidus |
| 620000 | - |
- |
Fusarium solani |
| 650000 | - |
above 650000 Da | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeribacillus pallidus | Q0PIV8 | - |
- |
| Aeribacillus pallidus Dac521 | Q0PIV8 | - |
- |
| Arabidopsis thaliana | P32961 | - |
- |
| Arthrobacter sp. | - |
strain J1 | - |
| Aspergillus niger | A9QXE0 | - |
- |
| Aspergillus niger K10 | A9QXE0 | - |
- |
| Bradyrhizobium japonicum | - |
- |
- |
| Bradyrhizobium japonicum USDA 110 | - |
- |
- |
| Brassica napus | - |
- |
- |
| Fusarium oxysporum f. sp. melonis | - |
- |
- |
| Fusarium solani | - |
- |
- |
| Fusarium solani IMI 196840 | - |
- |
- |
| Fusarium solani O1 | - |
- |
- |
| Rhodococcus rhodochrous | - |
- |
- |
| Rhodococcus rhodochrous ATCC 39484 | - |
- |
- |
| Rhodococcus rhodochrous PA-34 | - |
- |
- |
| Rhodococcus sp. | - |
- |
- |
| Rhodococcus sp. NCIMB 11215 | - |
- |
- |
| Rhodococcus sp. NCIMB 11216 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-furanocarbonnitrile + H2O | 171% activity compared to benzonitrile | Rhodococcus rhodochrous | 2-furanocarboxylic acid + NH3 | - |
? | |
| 2-furanocarbonnitrile + H2O | 171% activity compared to benzonitrile | Rhodococcus rhodochrous ATCC 39484 | 2-furanocarboxylic acid + NH3 | - |
? | |
| 2-furanocarbonnitrile + H2O | 171% activity compared to benzonitrile | Rhodococcus rhodochrous PA-34 | 2-furanocarboxylic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 174.8% activity compared to benzonitrile | Rhodococcus sp. | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 841.7% activity compared to benzonitrile | Rhodococcus sp. | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 174.8% activity compared to benzonitrile | Rhodococcus sp. NCIMB 11216 | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 841.7% activity compared to benzonitrile | Rhodococcus sp. NCIMB 11216 | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 174.8% activity compared to benzonitrile | Rhodococcus sp. NCIMB 11215 | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 841.7% activity compared to benzonitrile | Rhodococcus sp. NCIMB 11215 | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3-phenylpropionitrile + H2O | the enzyme has 270times more activity with 3-phenylpropionitrile than that observed with benzonitrile | Arabidopsis thaliana | 3-phenylpropionic acid + NH3 | - |
? | |
| 4-cyanopyridine + 2 H2O | 130% activity compared to benzonitrile | Fusarium solani | 4-pyridinecarboxylic acid + NH3 | - |
? | |
| 4-cyanopyridine + 2 H2O | 130% activity compared to benzonitrile | Fusarium solani IMI 196840 | 4-pyridinecarboxylic acid + NH3 | - |
? | |
| 4-cyanopyridine + 2 H2O | 130% activity compared to benzonitrile | Fusarium solani O1 | 4-pyridinecarboxylic acid + NH3 | - |
? | |
| 4-cyanopyridine + H2O | 410.7% activity compared to benzonitrile | Aspergillus niger | pyridine 4-carboxylic acid + NH3 | - |
? | |
| 4-cyanopyridine + H2O | 410.7% activity compared to benzonitrile | Aspergillus niger K10 | pyridine 4-carboxylic acid + NH3 | - |
? | |
| 4-tolunitrile + H2O | 125% activity compared to benzonitrile | Arthrobacter sp. | 4-methylbenzoic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 22.4% activity compared to benzonitrile | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 35% activity compared to benzonitrile | Fusarium oxysporum f. sp. melonis | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 6.6% activity compared to benzonitrile | Fusarium solani | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 6.6% activity compared to benzonitrile | Fusarium solani IMI 196840 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 6.6% activity compared to benzonitrile | Fusarium solani O1 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 22.4% activity compared to benzonitrile | Rhodococcus rhodochrous ATCC 39484 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 22.4% activity compared to benzonitrile | Rhodococcus rhodochrous PA-34 | acrylic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Bradyrhizobium japonicum | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Arabidopsis thaliana | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Arthrobacter sp. | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Rhodococcus sp. | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Fusarium solani | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Aspergillus niger | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Aeribacillus pallidus | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Fusarium oxysporum f. sp. melonis | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Bradyrhizobium japonicum USDA 110 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Rhodococcus sp. NCIMB 11216 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Fusarium solani IMI 196840 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Fusarium solani O1 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Aeribacillus pallidus Dac521 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Rhodococcus rhodochrous ATCC 39484 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Aspergillus niger K10 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Rhodococcus sp. NCIMB 11215 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 100% activity | Rhodococcus rhodochrous PA-34 | benzoic acid + NH3 | - |
? | |
| crotonitrile + H2O | 80.3% activity compared to benzonitrile | Aeribacillus pallidus | crotonic acid + NH3 | - |
? | |
| crotonitrile + H2O | 80.3% activity compared to benzonitrile | Aeribacillus pallidus Dac521 | crotonic acid + NH3 | - |
? | |
| hydrocinnamonitrile + H2O | the enzyme has 431times more activity with hydrocinnamonitrile than that observed with benzonitrile | Bradyrhizobium japonicum | hydrocinnamic acid + NH3 | - |
? | |
| hydrocinnamonitrile + H2O | the enzyme has 431times more activity with hydrocinnamonitrile than that observed with benzonitrile | Bradyrhizobium japonicum USDA 110 | hydrocinnamic acid + NH3 | - |
? | |
| phenylpropionitrile + 2 H2O | - |
Brassica napus | phenylpropionic acid + NH3 | - |
? | |
| propionitrile + H2O | - |
Rhodococcus sp. | propionic acid + NH3 | - |
? | |
| propionitrile + H2O | - |
Rhodococcus sp. NCIMB 11216 | propionic acid + NH3 | - |
? | |
| propionitrile + H2O | - |
Rhodococcus sp. NCIMB 11215 | propionic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodecamer | 10 * 34500 | Bradyrhizobium japonicum |
| homodecamer or homododecamer | 10 * 38500 or 12 * 38500 | Aspergillus niger |
| homodecamer or homoundecamer | 10 * 38000 or 11 * 38000 | Brassica napus |
| homododecamer | 12 * 45000 | Rhodococcus sp. |
| homododecamer | 12 * 45800 | Rhodococcus sp. |
| homododecamer | 14 * 38000 | Arabidopsis thaliana |
| homooctamer | 8 * 76000 | Fusarium solani |
| homotetradecamer | 14 * 37000 | Fusarium oxysporum f. sp. melonis |
| homotetradecamer | 14 * 40000 | Rhodococcus rhodochrous |
| homotetradecamer | 14 * 40000 | Fusarium solani |
| homotetradecamer | 14 * 41000 | Aeribacillus pallidus |
| monomer | 1 * 30000 | Arthrobacter sp. |
| monomer | 1 * 45000 | Rhodococcus rhodochrous |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NIT1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhodococcus sp. |
| 35 | - |
- |
Rhodococcus rhodochrous |
| 35 | - |
- |
Brassica napus |
| 35 | - |
- |
Arabidopsis thaliana |
| 40 | 45 | - |
Fusarium solani |
| 40 | - |
- |
Rhodococcus rhodochrous |
| 40 | - |
- |
Arthrobacter sp. |
| 40 | - |
- |
Fusarium oxysporum f. sp. melonis |
| 45 | - |
- |
Bradyrhizobium japonicum |
| 45 | - |
- |
Aspergillus niger |
| 65 | - |
- |
Aeribacillus pallidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | - |
Fusarium oxysporum f. sp. melonis |
| 7 | 8 | - |
Bradyrhizobium japonicum |
| 7 | 9.5 | - |
Rhodococcus sp. |
| 7.5 | - |
- |
Rhodococcus rhodochrous |
| 7.6 | - |
- |
Aeribacillus pallidus |
| 7.8 | 9.1 | - |
Fusarium solani |
| 8 | - |
- |
Rhodococcus sp. |
| 8 | - |
- |
Fusarium solani |
| 8 | - |
- |
Aspergillus niger |
| 8.5 | - |
- |
Arthrobacter sp. |
| 9 | - |
- |
Brassica napus |
| 9 | - |
- |
Arabidopsis thaliana |
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