Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.1 extracted from

  • Kaul, P.; Banerjee, U.C.
    Predicting enzyme behavior in nonconventional media: correlating nitrilase function with solvent properties (2008), J. Ind. Microbiol. Biotechnol., 35, 713-720.
    View publication on PubMed

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15% Alcaligenes faecalis
dimethyl acetamide extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced at 5% Alcaligenes faecalis
dimethyl formamide extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-25% Alcaligenes faecalis
dimethyl sulfoxide signifantly enhances the synthetic potential of the enzyme as well as its enantioselectivity. Extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5–25% Alcaligenes faecalis
dioxane extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-10% Alcaligenes faecalis
Ethanol extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20% Alcaligenes faecalis
isopropanol extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15% Alcaligenes faecalis
Methanol extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20% Alcaligenes faecalis
additional information addition of organic solvent up to 10%-20% leads to an enhancement in reaction rate, any further increase beyond the critical concentration in the latter leads to the decrease in catalytic efficiency of the enzyme. The solvent dielectric constant shows a linear correlation with the critical concentration of the solvent used and the extent of nitrile hydrolysis. Unlike alcohols, the reaction rate in case of aprotic solvents can be linearly correlated to solvent log P. The affinity of the enzyme for its substrate is highly dependent upon the aprotic solvent used Alcaligenes faecalis
N-methyl pyrrolidone addition results in decreased rate of hydrolysis Alcaligenes faecalis
n-propanol extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15% Alcaligenes faecalis
N-vinyl pyrrolidone addition results in decreased rate of hydrolysis Alcaligenes faecalis
Pyridine addition results in decreased rate of hydrolysis Alcaligenes faecalis
tetrahydrofuran addition results in decreased rate of hydrolysis Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis MTCC 126
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (R,S)-mandelonitrile + 4 H2O
-
Alcaligenes faecalis (R)-mandelic acid + NH3 + (S)-mandelonitrile
-
?
2 (R,S)-mandelonitrile + 4 H2O
-
Alcaligenes faecalis MTCC 126 (R)-mandelic acid + NH3 + (S)-mandelonitrile
-
?