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Literature summary for 3.5.5.1 extracted from

  • Jenrich, R.; Trompetter, I.; Bak, S.; Olsen, C.E.; Moller, B.L.; Piotrowski, M.
    Evolution of heteromeric nitrilase complexes in Poaceae with new functions in nitrile metabolism (2007), Proc. Natl. Acad. Sci. USA, 104, 18848-18853.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 CodonPlus (DE3) RIL cells Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 recombinant isozyme NIT4A, SDS-PAGE Sorghum bicolor
42000
-
 recombinant isozyme NIT4B2, SDS-PAGE Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA column chromatography Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylacetonitrile + 2 H2O substrate for isozyme complex NIT4A/B2 Sorghum bicolor phenylacetate + NH3
-
 ?
4-hydroxyphenylacetonitrile + H2O substrate for isozyme complex NIT4A/B2 Sorghum bicolor 4-hydroxyphenylacetic acid + NH3
-
 ?
6-heptenenitrile + H2O substrate for isozyme NIT4A/B2 Sorghum bicolor 6-heptenoic acid + NH3
-
 ?
indole-3-acetonitrile + H2O substrate for isozyme complex NIT4A/B2 Sorghum bicolor indol-3-acetic acid + NH3
-
 ?
additional information recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile Sorghum bicolor ?
-
 ?

Synonyms

Synonyms Comment Organism
NIT4A/B1 active isozyme complex Sorghum bicolor
NIT4A/B2 actice isozyme complex Sorghum bicolor