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Literature summary for 3.5.5.1 extracted from

  • Ishikawa, T.; Okazaki, K.; Kuroda, H.; Itoh, K.; Mitsui, T.; Hori, H.
    Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development (2007), Mol. Plant Pathol., 8, 623-637.
    View publication on PubMed
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Brassica rapa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 3-Phenylpropionitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
0.34
-
 4-phenylbutyronitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
0.35
-
 heptanenitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
2.12
-
 indole-3-acetonitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
2.49
-
 allyl cyanide isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa

Organism

Organism UniProt Comment Textmining
Brassica rapa A3QYW3 NIT-T2; cultivar Natsumaki
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose Fast Flow column chromatography and Sephadex G-15 gel filtration Brassica rapa

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Brassica rapa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phenylpropionitrile + H2O
-
 Brassica rapa 3-phenylpropionic acid + NH3
-
 ?
4-phenylbutyronitrile + H2O
-
 Brassica rapa 4-phenylbutyric acid + NH3
-
 ?
allyl cyanide + H2O
-
 Brassica rapa prop-2-enoic acid + NH3
-
 ?
heptanenitrile + H2O
-
 Brassica rapa heptanoic acid + NH3
-
 ?
indole-3-acetonitrile + H2O poor substrate Brassica rapa indole-3-acetic acid + NH3
-
 ?
additional information 3-cyano-L-alanine is no substrate Brassica rapa ?
-
 ?

Synonyms

Synonyms Comment Organism
NIT-T2 isozyme Brassica rapa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 indole-3-acetonitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
15.8
-
 4-phenylbutyronitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
19.8
-
 allyl cyanide isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
40.8
-
 heptanenitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
79.7
-
 3-Phenylpropionitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa