Literature summary for 3.5.5.1 extracted from

Kriechbaumer, V.; Park, W.J.; Piotrowski, M.; Meeley, R.B.; Gierl, A.; Glawischnig, E.
Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis (2007), J. Exp. Bot., 58, 4225-4233.

Search for more literature for 3.5.5.1

Activating Compound

Activating Compound	Comment	Organism	Structure
indole-3-acetonitrile	induces protein expression	Zea mays
additional information	a 2fold induction by light is observed	Zea mays

Inhibitors

Inhibitors	Comment	Organism	Structure
jasmonic acid	application of jasmonic acid represses protein expression	Zea mays
additional information	gibberellic acid, kinetin, abscisic acid, or epi-brassinolide have no obvious influence on activity	Zea mays

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.9	-	indole-3-acetonitrile	-	Zea mays

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	line B73	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration	Zea mays

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Zea mays	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
indole-3-acetonitrile + H2O	preferred substrate of NIT2	Zea mays	indole-3-acetic acid + NH3	-	?
additional information	NIT2 does not use 3-cyano-L-alanine as a substrate, however, turnover of 3-cyano-L-alanine function is acquired when NIT2 forms heteromers with the homologue NIT1	Zea mays	?	-	?

Synonyms

Synonyms	Comment	Organism
NIT2	-	Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.13	-	indole-3-acetonitrile	-	Zea mays

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Release 2023.1 (January 2023)