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Literature summary for 3.5.5.1 extracted from

  • Mukherjee, C.; Zhu, D.; Biehl, E.R.; Hua, L.
    Exploring the synthetic applicability of a cyanobacterium nitrilase as catalyst for nitrile hydrolysis (2006), Eur. J. Org. Chem., 23, 5238-5242.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and gel filtration Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(methylthio)acetonitrile + H2O
-
Synechocystis sp. (methylthio)acetic acid + NH3
-
?
2 phenylacetonitrile + H2O
-
Synechocystis sp. 2-phenylacetate + NH3
-
?
2-phenylpropionitrile + 2 H2O
-
Synechocystis sp. 2-phenylpropionic acid + NH3
-
?
3-phenylpropionitrile + H2O
-
Synechocystis sp. 3-phenylpropionic acid + NH3
-
?
4-phenylbutyronitrile + H2O
-
Synechocystis sp. 4-phenylbutyric acid + NH3
-
?
benzonitrile + 2 H2O
-
Synechocystis sp. benzoic acid + NH3
-
?
mandelonitrile + 2 H2O
-
Synechocystis sp. mandelic acid + NH3
-
?
additional information does not convert 2-phenylbutyronitrile Synechocystis sp. ?
-
?