Literature summary for 3.5.5.1 extracted from

Wu, S.; Fogiel, A.J.; Petrillo, K.L.; Jackson, R.E.; Parker, K.N.; DiCosimo, R.; Ben-Bassat, A.; OKeefe, D.P.; Payne, M.S.
Protein engineering of nitrilase for chemoenzymatic production of glycolic acid (2007), Biotechnol. Bioeng., 99, 717-720.

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Protein Variants

Protein Variants	Comment	Organism
F168I/L201E	4.6fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
F168K	3.1fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
F168M	3fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
F168T	1.7fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
F168T/L201Q	3.1fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
F168V	4.1fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
F168V/L201N	15.3fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201A	1.9fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201G	1.5fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201H	2.7fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201K	3.5fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201N	5.5fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201Q	4.9fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201S	2.2fold increased specific activity compared to the wild type enzyme	Acidovorax facilis
L201T	2fold increased specific activity compared to the wild type enzyme	Acidovorax facilis

Organism

Organism	UniProt	Comment	Textmining
Acidovorax facilis	-	-	-
Acidovorax facilis 72W	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzonitrile + 2 H2O	-	Acidovorax facilis	benzoic acid + NH3	-	?
benzonitrile + 2 H2O	-	Acidovorax facilis 72W	benzoic acid + NH3	-	?
glycolonitrile + H2O	-	Acidovorax facilis	ammonium glycolate + ?	-	?
glycolonitrile + H2O	-	Acidovorax facilis 72W	ammonium glycolate + ?	-	?

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Release 2023.1 (January 2023)