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Literature summary for 3.5.5.1 extracted from

  • Vorwerk, S.; Biernacki, S.; Hillebrand, H.; Janzik, I.; Muller, A.; Weiler, E.W.; Piotrowski, M.
    Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster (2001), Planta, 212, 508-516.
    View publication on PubMed
Search for more literature for 3.5.5.1

Protein Variants

Protein Variants Comment Organism
C179A site-directed mutagenesis, completely inactive mutant, determined with substrate indole-3-acetonitrile Arabidopsis thaliana
C179N site-directed mutagenesis, completely inactive mutant, determined with substrate indole-3-acetonitrile Arabidopsis thaliana
C180A site-directed mutagenesis, completely inactive mutant, determined with substrate indole-3-acetonitrile Arabidopsis thaliana
C180N site-directed mutagenesis, completely inactive mutant, determined with substrate indole-3-acetonitrile Arabidopsis thaliana
C186A site-directed mutagenesis, completely inactive mutant, determined with substrate indole-3-acetonitrile Arabidopsis thaliana
C186N site-directed mutagenesis, completely inactive mutant, determined with substrate indole-3-acetonitrile Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 phenylbutyronitrile pH 8.0, 30°C, isozyme NIT3 Arabidopsis thaliana
0.08
-
 phenylbutyronitrile pH 8.0, 30°C, isozyme NIT1 Arabidopsis thaliana
0.09
-
 phenylbutyronitrile pH 8.0, 30°C, isozyme NIT2 Arabidopsis thaliana
0.14
-
 phenylacetonitrile pH 8.0, 30°C, isozyme NIT1 Arabidopsis thaliana
0.22
-
 phenylpropionitrile pH 8.0, 30°C, isozyme NIT1 Arabidopsis thaliana
0.23
-
 phenylacetonitrile pH 8.0, 30°C, isozyme NIT3 Arabidopsis thaliana
0.42
-
 phenylpropionitrile pH 8.0, 30°C, isozyme NIT3 Arabidopsis thaliana
0.43
-
 phenylpropionitrile pH 8.0, 30°C, isozyme NIT2 Arabidopsis thaliana
0.6
-
 allyl cyanide pH 8.0, 30°C, isozyme NIT1 Arabidopsis thaliana
0.88
-
 (methylthio)acetonitrile pH 8.0, 30°C, isozyme NIT1 Arabidopsis thaliana
1
-
 phenylacetonitrile pH 8.0, 30°C, isozyme NIT2 Arabidopsis thaliana
1.09
-
 (methylthio)acetonitrile pH 8.0, 30°C, isozyme NIT2 Arabidopsis thaliana
1.31
-
 (methylthio)acetonitrile pH 8.0, 30°C, isozyme NIT3 Arabidopsis thaliana
2.09
-
 allyl cyanide pH 8.0, 30°C, isozyme NIT3 Arabidopsis thaliana
6.27
-
 allyl cyanide pH 8.0, 30°C, isozyme NIT2 Arabidopsis thaliana
7.4
-
 indole-3-acetonitrile pH 8.0, 30°C, isozyme NIT2 Arabidopsis thaliana
11.1
-
 indole-3-acetonitrile pH 8.0, 30°C, isozyme NIT1 Arabidopsis thaliana
30.1
-
 indole-3-acetonitrile pH 8.0, 30°C, isozyme NIT3 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P32961 isozyme NIT1
-
Arabidopsis thaliana P32962 isozyme NIT2
-
Arabidopsis thaliana P46010 isozyme NIT3
-

Reaction

Reaction Comment Organism Reaction ID
a nitrile + 2 H2O = a carboxylate + NH3 Cys179 is essential for catalytic activity Arabidopsis thaliana
a
a nitrile + 2 H2O = a carboxylate + NH3 Cys186 is essential for catalytic activity Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
embryo predominant isoform NIT2 in developing embryos Arabidopsis thaliana
-
seed predominant isoform NIT2 in germinating seeds Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(methylthio)acetonitrile + H2O
-
 Arabidopsis thaliana 2-(methylthio)acetic acid + NH3
-
 ?
(methylthio)acetonitrile + H2O
-
 Arabidopsis thaliana (methylthio)acetic acid + NH3
-
 ?
(phenylthio)acetonitrile + H2O
-
 Arabidopsis thaliana 2-(phenylthio)acetic acid + NH3
-
 ?
1-(cyanoacetyl)pyrrolidine + H2O low activity Arabidopsis thaliana ? + NH3
-
 ?
2-cyanopyridine + 2 H2O low activity Arabidopsis thaliana pyridine 2-carboxylic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O low activity Arabidopsis thaliana pyridine 3-carboxylic acid + NH3
-
 ?
3-hydroxypropionitrile + H2O very low activity Arabidopsis thaliana 3-hydroxypropanoate + NH3
-
 ?
3-hydroxypropionitrile + H2O low activity Arabidopsis thaliana 3-hydroxypropanoate + NH3
-
 ?
4-hydroxybenzylcyanide + H2O low activity Arabidopsis thaliana 4-hydroxybenzoic acid + NH3
-
 ?
4-phenylbutyronitrile + H2O
-
 Arabidopsis thaliana 4-phenylbutyric acid + NH3
-
 ?
allyl cyanide + H2O
-
 Arabidopsis thaliana prop-2-enoic acid + NH3
-
 ?
allyl cyanide + H2O high activity Arabidopsis thaliana prop-2-enoic acid + NH3
-
 ?
chloroacetonitrile + H2O low activity Arabidopsis thaliana chloroacetic acid + NH3
-
 ?
cyclopropanecarbonitrile + H2O
-
 Arabidopsis thaliana cyclopropanecarboxylic acid + NH3
-
 ?
indole-3-acetonitrile + H2O low activity Arabidopsis thaliana indole-3-acetic acid + NH3
-
 ?
additional information broad substrate specificity, overview Arabidopsis thaliana ?
-
 ?
additional information isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification Arabidopsis thaliana ?
-
 ?
phenylacetonitrile + 2 H2O
-
 Arabidopsis thaliana phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O low activity Arabidopsis thaliana phenylacetic acid + NH3
-
 ?
phenylbutyronitrile + H2O
-
 Arabidopsis thaliana phenylbutyric acid + NH3
-
 ?
phenylbutyronitrile + H2O high activity Arabidopsis thaliana phenylbutyric acid + NH3
-
 ?
phenylpropionitrile + 2 H2O best substrate Arabidopsis thaliana phenylpropionic acid + NH3
-
 ?
propionitrile + H2O
-
 Arabidopsis thaliana propionic acid + NH3
-
 ?
propionitrile + H2O low activity Arabidopsis thaliana propionic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NIT2
-
 Arabidopsis thaliana
NIT3
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 with substrate indole-3-acetonitrile Arabidopsis thaliana
30
-
 about Arabidopsis thaliana
35 40 with substrate phenylpropionitrile Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH optimum at about neutral pH Arabidopsis thaliana