Application | Comment | Organism |
---|---|---|
synthesis | nitrilase products are intermediates in the synthesis of nylon | Comamonas testosteroni |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | Comamonas testosteroni | - |
acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | Comamonas testosteroni | low activity | benzoic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q59329 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Comamonas testosteroni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | nitA gene | Comamonas testosteroni | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
68 | - |
- |
Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | - |
Comamonas testosteroni | acrylic acid + NH3 | - |
? | |
adiponitrile + H2O | preferred substrate, specific for aliphatic nitriles | Comamonas testosteroni | adipic acid + NH3 | - |
? | |
adiponitrile + H2O | no formation of amide | Comamonas testosteroni | adipic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | low activity | Comamonas testosteroni | benzoic acid + NH3 | - |
? | |
cyano-5-valeramide + H2O | - |
Comamonas testosteroni | valeramide + NH3 | - |
? | |
cyano-5-valeric acid + H2O | - |
Comamonas testosteroni | valeric acid + NH3 | - |
? | |
propionitrile + H2O | low activity | Comamonas testosteroni | propionic acid + NH3 | - |
? |