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Literature summary for 3.5.5.1 extracted from

  • Harper, D.B.
    Microbial metabolism of aromatic nitriles. Enzymology of C-N cleavage by Nocardia sp. (Rhodococcus group) N.C.I.B. 11216 (1977), Biochem. J., 165, 309-319.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.1

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoic acid complete inhibition at 0.0005 mM Nocardia sp.
Ag+ complete inhibition at 0.01 mM Nocardia sp.
Co2+ 97% inhibition at 0.1 mM Nocardia sp.
Cu2+ complete inhibition at 0.01 mM Nocardia sp.
Fe2+ 37% inhibition at 0.001 mM Nocardia sp.
Hg2+ complete inhibition at 0.01 mM Nocardia sp.
iodoacetamide complete inhibition at 0.5 mM Nocardia sp.
Mn2+ complete inhibition at 0.1 mM Nocardia sp.
N-ethylmaleimide complete inhibition at 0.005 mM Nocardia sp.
Phenylmercuriacetate complete inhibition at 0.005 mM Nocardia sp.
Zn2+ complete inhibition at 0.01 mM Nocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 Benzonitrile
-
 Nocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 substrate, temperature and pH dependent association of 12 subunits, 12 * 45000, SDS-PAGE Nocardia sp.
560000
-
 gel filtration, substrate catalysed association of inactive monomers Nocardia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + 2 H2O Nocardia sp.
-
 nicotinic acid + NH3
-
 ?
benzonitrile + 2 H2O Nocardia sp. specific for nitrile groups directly attached to the benzene ring benzoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Nocardia sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture use of benzonitrile as a sole source of nitrogen and carbon Nocardia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.7
-
-
 Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-benzodinitrile + H2O
-
 Nocardia sp. benzoic acid + NH3
-
 ?
2-fluorobenzonitrile + H2O
-
 Nocardia sp. 2-fluorobenzoic acid + NH3
-
 ?
3-bromobenzonitrile + H2O best substrate Nocardia sp. 3-bromobenzoic acid + NH3
-
 ?
3-chlorobenzonitrile + H2O
-
 Nocardia sp. 3-chlorobenzoic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Nocardia sp. nicotinic acid + NH3
-
 ?
3-fluorobenzonitrile + H2O
-
 Nocardia sp. 3-fluorobenzoic acid + NH3
-
 ?
3-nitrobenzonitrile + H2O
-
 Nocardia sp. 3-nitrobenzoic acid + NH3
-
 ?
3-toluonitrile + H2O
-
 Nocardia sp. NH3 + 3-methylbenzoate
-
 ?
4-bromobenzonitrile + H2O
-
 Nocardia sp. 4-bromobenzoic acid + NH3
-
 ?
4-chlorobenzonitrile + H2O
-
 Nocardia sp. 4-chlorobenzoic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O
-
 Nocardia sp. 4-pyridinecarboxylic acid + NH3
-
 ?
4-fluorobenzonitrile + H2O
-
 Nocardia sp. 4-fluorobenzoic acid + NH3
-
 ?
4-toluonitrile + H2O
-
 Nocardia sp. NH3 + 4-methylbenzoate
-
 ?
benzonitrile + 2 H2O specific for nitrile groups directly attached to the benzene ring Nocardia sp. benzoic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
dodecamer substrate, temperature and pH dependent association of 12 subunits, 12 * 45000, SDS-PAGE Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Nocardia sp.