Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.1 extracted from

  • Bandypadhyay, A.K.; Nagasawa, T.; Asano, Y.; Fujishiro, K.; Tani, Y.; Yamada, H.
    Purification and characterization of benzonitrilases from Arthrobacter sp. strain J-1 (1986), Appl. Environ. Microbiol., 51, 302-306.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.1

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 91-100% inhibition at 0.1 mM Arthrobacter sp.
Ag+ complete inhibition at 0.1 mM Arthrobacter sp.
CaCl2 inhibition at 50 mM Arthrobacter sp.
Cu2+ benzonitrilase A: 30% inhibition at 0.1 mM, benzonitrilase B: 83% inhibition at 0.1 mM Arthrobacter sp.
Hg2+ complete inhibition at 0.1 mM Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
 Benzonitrile benzonitrilase B Arthrobacter sp.
6.7
-
 Benzonitrile benzonitrilase A Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration, benzonitrilase B Arthrobacter sp.
30000
-
 gel filtration, benzonitrilase A Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. J-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
benzonitrilase A, to homogeneity, partial for benzonitrilase B Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture use of benzonitrile as a sole source of nitrogen and carbon Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
 benzonitrilase B Arthrobacter sp.
1.31
-
 benzonitrilase A Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorobenzonitrile + H2O
-
 Arthrobacter sp. 4-chlorobenzoic acid + NH3
-
 ?
4-chlorobenzonitrile + H2O
-
 Arthrobacter sp. J-1 4-chlorobenzoic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O best substrate Arthrobacter sp. 4-pyridinecarboxylic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O best substrate Arthrobacter sp. J-1 4-pyridinecarboxylic acid + NH3
-
 ?
4-toluonitrile + H2O
-
 Arthrobacter sp. NH3 + 4-methylbenzoate
-
 ?
4-toluonitrile + H2O
-
 Arthrobacter sp. J-1 NH3 + 4-methylbenzoate
-
 ?
benzonitrile + 2 H2O
-
 Arthrobacter sp. benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Arthrobacter sp. J-1 benzoic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, benzonitrilase A, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
benzonitrilase A
-
 Arthrobacter sp.
benzonitrilase B
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 benzonitrilase B Arthrobacter sp.
40
-
 benzonitrilase A Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 benzonitrilase B: gradually degraded above Arthrobacter sp.
45
-
 benzonitrilase A, stable up to Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 benzonitrilase B Arthrobacter sp.
8.5
-
 benzonitrilase A Arthrobacter sp.