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Literature summary for 3.5.5.1 extracted from

  • Stevenson, D.E.; Feng, R.; Dumas, F.; Groleau, D.; Mihoc, A.; Storer, A.C.
    Mechanistic and structural studies on Rhodococcus ATCC 39484 nitrilase (1992), Biotechnol. Appl. Biochem., 15, 283-302.
    View publication on PubMed
Search for more literature for 3.5.5.1

Application

Application Comment Organism
synthesis preparing carboxylic acids from readily available nitrile analogues Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 1 mM Rhodococcus sp.
benzaldehyde nearly complete inhibition at 10 mM Rhodococcus sp.
benzyl bromide strong inhibition at 10 mM Rhodococcus sp.
benzylamine 65% inhibition at 10 mM Rhodococcus sp.
Hg2+ nearly complete inactivation at 1 mM Rhodococcus sp.
phenylacetonitrile 50% inhibition at 10 mM Rhodococcus sp.
phenylmethylsulfonyl fluoride 40% inhibition at 1 mM Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 3-chlorobenzonitrile
-
 Rhodococcus sp.
2
-
 4-nitrobenzonitrile
-
 Rhodococcus sp.
2.4
-
 3-methylbenzonitrile
-
 Rhodococcus sp.
2.6
-
 3-cyanobenzonitrile
-
 Rhodococcus sp.
2.7
-
 3-Methoxybenzonitrile
-
 Rhodococcus sp.
3.5
-
 3-aminobenzonitrile
-
 Rhodococcus sp.
3.8
-
 Benzonitrile
-
 Rhodococcus sp.
4.8
-
 3-Nitrobenzonitrile
-
 Rhodococcus sp.
8
-
 4-aminobenzonitrile
-
 Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
 1 * 41500, SDS-PAGE Rhodococcus sp.
46000
-
 gel filtration, aggregation to 560 kDa in the presence of benzonitrile Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + 2 H2O Rhodococcus sp.
-
 nicotinic acid + NH3
-
 ?
acrylonitrile + H2O Rhodococcus sp.
-
 acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O Rhodococcus sp.
-
 benzoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
 strain ATCC 39484
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Rhodococcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 phenylacetonitrile + 3 H2O
-
 Rhodococcus sp. phenylacetamide + phenylacetate + NH3 not completely converted to acid, 2% of the product is phenylacetamide ?
2-furanocarbonitrile + H2O best substrate Rhodococcus sp. furan-2-carboxylic acid + NH3
-
 ?
3-aminobenzonitrile + H2O
-
 Rhodococcus sp. 3-aminobenzoic acid + NH3
-
 ?
3-chlorobenzonitrile + H2O
-
 Rhodococcus sp. 3-chlorobenzoic acid + NH3
-
 ?
3-cyanobenzonitrile + H2O
-
 Rhodococcus sp. 3-cyanobenzoic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Rhodococcus sp. nicotinic acid + NH3
-
 ?
3-methoxybenzonitrile + H2O
-
 Rhodococcus sp. 3-methoxybenzoic acid + NH3
-
 ?
3-nitrobenzonitrile + H2O
-
 Rhodococcus sp. 3-nitrobenzoic acid + NH3
-
 ?
3-toluonitrile + H2O
-
 Rhodococcus sp. NH3 + 3-methylbenzoate
-
 ?
4-aminobenzonitrile + H2O
-
 Rhodococcus sp. 4-aminobenzoic acid + NH3
-
 ?
4-nitrobenzonitrile + H2O
-
 Rhodococcus sp. 4-nitrobenzoic acid + NH3
-
 ?
acrylonitrile + H2O
-
 Rhodococcus sp. acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Rhodococcus sp. benzoic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41500, SDS-PAGE Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 irreversible inactivation by preincubation above Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 active within Rhodococcus sp.
5.5 8 stable between Rhodococcus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10
-
 phenylacetonitrile
-
 Rhodococcus sp.