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Literature summary for 3.5.5.1 extracted from

  • Kobayashi, M.; Komeda, H.; Yanaka, N.; Nagasawa, T.; Yamada, H.
    Nitrilase from Rhodococcus rhodochrous J1. Sequencing and overexpression of the gene and identification of an essential cysteine residue (1992), J. Biol. Chem., 267, 20746-20751.
    View publication on PubMed
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus rhodochrous

Protein Variants

Protein Variants Comment Organism
C165A no activity Rhodococcus rhodochrous
C165S no activity Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) complete loss of activity due to modification of Cys165 Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzonitrile + 2 H2O Rhodococcus rhodochrous
-
 benzoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

Purification (Comment) Organism
highly purified, recombinant enzyme Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.04
-
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzonitrile + 2 H2O
-
 Rhodococcus rhodochrous benzoic acid + NH3
-
 ?