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Literature summary for 3.5.5.1 extracted from

  • Nagawasa, T.; Wieser, M.; Nakamura, T.; Iwahara, H.; Yoshida, T.; Gekko, K.
    Nitrilase of Rhodococcus rhodochrous J1. Conversion into active form by subunit association (2000), Eur. J. Biochem., 267, 138-144.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition at 0.25 mM, also inhibition of enzyme association to decamers Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE, no hydrolysis of acrylonitrile Rhodococcus rhodochrous
40000
-
10 * 40000, SDS-PAGE, hydrolysis of both benzonitrile and acrylonitrile after recovery conditions for acrylonitrile hydrolysis Rhodococcus rhodochrous
80000
-
gel filtration, only active on benzonitrile Rhodococcus rhodochrous
412000
-
sedimentation equilibrium analysis, after recovery conditions for hydrolysis of acrylonitrile Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + 2 H2O Rhodococcus rhodochrous lower activity for the purified enzyme compared to the activity in resting cells nicotinic acid + NH3
-
?
acrylonitrile + H2O Rhodococcus rhodochrous only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate acrylic acid + NH3
-
?
benzonitrile + 2 H2O Rhodococcus rhodochrous best substrate benzoic acid + NH3
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2step chromatography Rhodococcus rhodochrous

Source Tissue

Source Tissue Comment Organism Textmining
cell culture induction of enzyme synthesis by epsilon-caprolactam- or isovaleronitrile-containing media Rhodococcus rhodochrous
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + 2 H2O lower activity for the purified enzyme compared to the activity in resting cells Rhodococcus rhodochrous nicotinic acid + NH3
-
?
acrylonitrile + H2O only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate Rhodococcus rhodochrous acrylic acid + NH3
-
?
benzonitrile + 2 H2O best substrate Rhodococcus rhodochrous benzoic acid + NH3
-
?
crotononitrile + H2O low activity Rhodococcus rhodochrous NH3 + (z)-but-2-en-1-ol
-
?
methacrylonitrile + H2O very low activity in resting cells and for the purified enzyme only after recovered activity by dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate Rhodococcus rhodochrous methylacrylic acid + NH3
-
?

Subunits

Subunits Comment Organism
decamer 10 * 40000, SDS-PAGE, hydrolysis of both benzonitrile and acrylonitrile after recovery conditions for acrylonitrile hydrolysis Rhodococcus rhodochrous
dimer 2 * 40000, SDS-PAGE, no hydrolysis of acrylonitrile Rhodococcus rhodochrous