Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D316R/D317R | the mutations increase affinity for substrate and deamination specificity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | Homo sapiens | the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently | uracil in single-stranded viral DNA + NH3 | - |
? | |
additional information | Homo sapiens | the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose resin column chromatography and DEAE FF column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AAAGAGAAAGAGAAACCCAAAGAGGAAAGGTGAGGAGAA-3' + H2O | the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently | Homo sapiens | 5'-AAAGAGAAAGAGAAACCUAAAGAGGAAAGGTGAGGAGAA-3' + NH3 | - |
? | |
cytosine in single-stranded viral DNA + H2O | the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently | Homo sapiens | uracil in single-stranded viral DNA + NH3 | - |
? | |
additional information | the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
A3G | - |
Homo sapiens |
APOBEC3G | - |
Homo sapiens |
apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G | - |
Homo sapiens |