Protein Variants | Comment | Organism |
---|---|---|
C243A/C321A/C356A | the mutation has no effect on localization, deamination, oligomerization, or HIV-1 Vif-deficient restriction capabilities. The mutant is only partially resistant to inhibitor MN256.0105, with recovered deamination efficiency of 19% | Homo sapiens |
C321A | the mutation has no effect on localization, deamination, oligomerization, or HIV-1 Vif-deficient restriction capabilities. The mutant is only partially resistant to inhibitor MN256.0105, with recovered deamination efficiency of 21% | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-amino-5-methyl-1,2,4-triazole-3-thiol | - |
Homo sapiens | |
4-[(4-bromobenzylidene)amino]-1,2,4-triazole-3-thiol | MN256.0105, 99% inhibition at 0.02 mM | Homo sapiens | |
4-[(4-methoxybenzylidene)amino]-5-methyl-1,2,4-triazole-3-thiol | - |
Homo sapiens | |
additional information | not inhibited by 4-amino-1,2,4-triazol-3-ol, 4-amino-5-methyl-1,2,4-triazol-3-ol, 6-(4-methoxyphenyl)-3-methyl-1,2,4-triazolo[3,4-b]-[1,3,4]thiadiazole, and 3-(benzylthio)-5-methyl-1,2,4-triazol-4-amine | Homo sapiens | |
N-(3-thio-5-methyl-1,2,4-triazol-4-yl)benzamide | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains a catalytic zinc atom | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | Homo sapiens | - |
uracil in single-stranded viral DNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | - |
Homo sapiens | uracil in single-stranded viral DNA + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A3G | - |
Homo sapiens |
APOBEC3G | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0039 | - |
at pH 7.4 and 37°C | Homo sapiens | 4-[(4-methoxybenzylidene)amino]-5-methyl-1,2,4-triazole-3-thiol | |
0.0043 | - |
at pH 7.4 and 37°C | Homo sapiens | 4-[(4-bromobenzylidene)amino]-1,2,4-triazole-3-thiol | |
0.0061 | - |
at pH 7.4 and 37°C | Homo sapiens | 4-amino-5-methyl-1,2,4-triazole-3-thiol | |
0.0082 | - |
at pH 7.4 and 37°C | Homo sapiens | N-(3-thio-5-methyl-1,2,4-triazol-4-yl)benzamide |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a single-stranded DNA cytosine deaminase that functions in innate immunity against retroviruses and retrotransposons. The enzyme can potently restrict virus infectivity factor-deficient HIV-1 replication by catalyzing excessive levels of G->A hypermutation. Sublethal levels of enzyme-catalyzed mutation may contribute to the high level of HIV-1 fitness and its incurable prognosis | Homo sapiens |