Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.8 extracted from

  • Rabinowitz, J.C.; Pricer, W.E.
    Purine fermentation by Clostridium cylindrosporum. V. Formiminoglycine (1956), J. Biol. Chem., 222, 537-554.
    View publication on PubMed
Search for more literature for 3.5.4.8

General Stability

General Stability Organism
unstable in absence of reducing agents, completely inactive without cysteine, cysteine, sodium hydrosulfite, glutathione, sodium thioglycolate and ascorbic acid stabilizes enzyme activity Clostridium cylindrosporum

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole
-
 Clostridium cylindrosporum
4-Hydroxy-1,2,3-triazole less effective inhibitor Clostridium cylindrosporum
Co2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum
EDTA
-
 Clostridium cylindrosporum
histamine less effective inhibitor Clostridium cylindrosporum
Mg2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum
Mn2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum
Zn2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 4-aminoimidazole
-
 Clostridium cylindrosporum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ restores activity of EDTA treated enzyme to 83% of the original activity Clostridium cylindrosporum
Fe2+ divalent cation required for activity Clostridium cylindrosporum
Mn2+ restores activity of EDTA treated enzyme to 60% of the original activity Clostridium cylindrosporum
Ni2+ restores activity of EDTA treated enzyme to 23% of the original activity Clostridium cylindrosporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminoimidazole + H2O Clostridium cylindrosporum
-
 formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?
4-aminoimidazole + H2O Clostridium cylindrosporum HC1
-
 formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?

Organism

Organism UniProt Comment Textmining
Clostridium cylindrosporum
-
-
-
Clostridium cylindrosporum HC1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium cylindrosporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
-
 Clostridium cylindrosporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoimidazole + H2O
-
 Clostridium cylindrosporum formiminoglycine + NH3 + unidentified product
-
 ?
4-aminoimidazole + H2O
-
 Clostridium cylindrosporum formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?
4-aminoimidazole + H2O
-
 Clostridium cylindrosporum HC1 formiminoglycine + NH3 + unidentified product
-
 ?
4-aminoimidazole + H2O
-
 Clostridium cylindrosporum HC1 formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in triethanolamine hydrochloride Clostridium cylindrosporum