BRENDA - Enzyme Database
show all sequences of 3.5.4.8

Purine fermentation by Clostridium cylindrosporum. V. Formiminoglycine

Rabinowitz, J.C.; Pricer, W.E.; J. Biol. Chem. 222, 537-554 (1956) View publication on PubMed

Data extracted from this reference:

General Stability
General Stability
Organism
unstable in absence of reducing agents, completely inactive without cysteine, cysteine, sodium hydrosulfite, glutathione, sodium thioglycolate and ascorbic acid stabilizes enzyme activity
Clostridium cylindrosporum
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-Amino-1,2,4-triazole
-
Clostridium cylindrosporum
4-Hydroxy-1,2,3-triazole
less effective inhibitor
Clostridium cylindrosporum
Co2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
EDTA
-
Clostridium cylindrosporum
histamine
less effective inhibitor
Clostridium cylindrosporum
Mg2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
Mn2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
Zn2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8
-
4-aminoimidazole
-
Clostridium cylindrosporum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
restores activity of EDTA treated enzyme to 83% of the original activity
Clostridium cylindrosporum
Fe2+
divalent cation required for activity
Clostridium cylindrosporum
Mn2+
restores activity of EDTA treated enzyme to 60% of the original activity
Clostridium cylindrosporum
Ni2+
restores activity of EDTA treated enzyme to 23% of the original activity
Clostridium cylindrosporum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-aminoimidazole + H2O
Clostridium cylindrosporum
-
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
Clostridium cylindrosporum
?
4-aminoimidazole + H2O
Clostridium cylindrosporum HC1
-
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
Clostridium cylindrosporum HC1
?
Organism
Organism
UniProt
Commentary
Textmining
Clostridium cylindrosporum
-
-
-
Clostridium cylindrosporum HC1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Clostridium cylindrosporum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
140
-
-
Clostridium cylindrosporum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum
formiminoglycine + NH3 + unidentified product
-
-
-
?
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
209717
Clostridium cylindrosporum
?
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum HC1
formiminoglycine + NH3 + unidentified product
-
-
-
?
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum HC1
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
209717
Clostridium cylindrosporum HC1
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
in triethanolamine hydrochloride
Clostridium cylindrosporum
General Stability (protein specific)
General Stability
Organism
unstable in absence of reducing agents, completely inactive without cysteine, cysteine, sodium hydrosulfite, glutathione, sodium thioglycolate and ascorbic acid stabilizes enzyme activity
Clostridium cylindrosporum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-Amino-1,2,4-triazole
-
Clostridium cylindrosporum
4-Hydroxy-1,2,3-triazole
less effective inhibitor
Clostridium cylindrosporum
Co2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
EDTA
-
Clostridium cylindrosporum
histamine
less effective inhibitor
Clostridium cylindrosporum
Mg2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
Mn2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
Zn2+
addition of 0.002 M reduces activity by 50% or more
Clostridium cylindrosporum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8
-
4-aminoimidazole
-
Clostridium cylindrosporum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
restores activity of EDTA treated enzyme to 83% of the original activity
Clostridium cylindrosporum
Fe2+
divalent cation required for activity
Clostridium cylindrosporum
Mn2+
restores activity of EDTA treated enzyme to 60% of the original activity
Clostridium cylindrosporum
Ni2+
restores activity of EDTA treated enzyme to 23% of the original activity
Clostridium cylindrosporum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-aminoimidazole + H2O
Clostridium cylindrosporum
-
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
Clostridium cylindrosporum
?
4-aminoimidazole + H2O
Clostridium cylindrosporum HC1
-
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
Clostridium cylindrosporum HC1
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium cylindrosporum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
140
-
-
Clostridium cylindrosporum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum
formiminoglycine + NH3 + unidentified product
-
-
-
?
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
209717
Clostridium cylindrosporum
?
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum HC1
formiminoglycine + NH3 + unidentified product
-
-
-
?
4-aminoimidazole + H2O
-
209717
Clostridium cylindrosporum HC1
formiminoglycine + NH3 + unidentified product
alkali-labile derivative of glycine, can be hydrolyzed to yield glycine
209717
Clostridium cylindrosporum HC1
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
in triethanolamine hydrochloride
Clostridium cylindrosporum
Other publictions for EC 3.5.4.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209717
Rabinowitz
Purine fermentation by Clostri ...
Clostridium cylindrosporum, Clostridium cylindrosporum HC1
J. Biol. Chem.
222
537-554
1956
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4
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4
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1
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1
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8
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1
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4
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2
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1
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1
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4
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1
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