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Literature summary for 3.5.4.6 extracted from
- AMP-deaminase from goldfish white muscle: regulatory properties and redistribution under exposure to high environmental oxygen level (2008), Fish Physiol. Biochem., 35, 443-452.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | strong activator, 1 mM ADP activates the enzyme by 3.2fold | Carassius auratus |  |
| ATP | strong activator, 1 mM ATP activates the enzyme by 1.7fold | Carassius auratus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 25% inhibition at 10 mM | Carassius auratus | |
| fluoride | inhibits AMPD by about 66% at 8 mM | Carassius auratus | |
| K+ | inhibitory at concentrations higher than 80 mM | Carassius auratus | |
| Mg2+ | 15% inhibition at 10 mM | Carassius auratus | |
| Na+ | inhibitory at concentrations higher than 80 mM | Carassius auratus | |
| phosphate | inhibits AMPD by about 66% at 8 mM | Carassius auratus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates AMPD at low concentrations, with maximal activation (1.9fold) at about 80 mM | Carassius auratus | |
| Na+ | activates AMPD at low concentrations, with maximal activation (1.3fold) at about 80 mM | Carassius auratus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
the SDS-PAGE shows that the enzyme is not electrophoretically homogenous, with two bands present at a molecular mass between about 55000 and 60000 Da | Carassius auratus |
| 60000 | - |
the SDS-PAGE shows that the enzyme is not electrophoretically homogenous, with two bands present at a molecular mass between about 55000 and 60000 Da | Carassius auratus |
| 160000 | - |
myosin-bound enzyme, SDS-PAGE | Carassius auratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carassius auratus | - |
goldfish | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphocellulose column chromatography | Carassius auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Carassius auratus | - |
| skeletal muscle | white skeletal muscle | Carassius auratus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2-4°C, following the purification process when stored in 1 M KCl, 1 month, the enzyme is highly stable showing virtually no change in activity | Carassius auratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + H2O | - |
Carassius auratus | IMP + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMP-aminohydrolase | - |
Carassius auratus |
| AMP-deaminase | - |
Carassius auratus |
| AmpD | - |
Carassius auratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Carassius auratus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | at pH values of 5.5, 7.0 and 7.5, AMPD shows 56, 80 and 54% of its optimal activity, respectively | Carassius auratus |
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Release 2023.1 (January 2023)
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