Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | muscle AMPD is activated by Mg ADP- | Lithobates sylvaticus | |
ATP | muscle AMPD is activated by Mg ATP2- | Lithobates sylvaticus | |
additional information | the percentage of bound AMPD activity increases from 20 to 35% with the transition to the frozen state | Lithobates sylvaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | very strong inhibitor | Lithobates sylvaticus | |
IMP | uniquely inhibits only the bound (phosphorylated) enzyme from muscle of frozen frogs | Lithobates sylvaticus | |
K+ | very weak inhibitor of free AMPD, but shows stronger effect on bound AMPD | Lithobates sylvaticus | |
Na+ | very weak inhibitor of free AMPD, but shows stronger effect on bound AMPD | Lithobates sylvaticus | |
NH3 | - |
Lithobates sylvaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithobates sylvaticus | - |
also known as Lithobates sylvaticus | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the reversible phosphorylation adjusts enzyme function for an optimal role in controlling cellular adenylate levels in ischemic frozen muscle | Lithobates sylvaticus |
Purification (Comment) | Organism |
---|---|
Sephadex G50 gel filtration | Lithobates sylvaticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Lithobates sylvaticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | - |
Lithobates sylvaticus | IMP + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-adenosine monophosphate deaminase | - |
Lithobates sylvaticus |
AMP deaminase | - |
Lithobates sylvaticus |
AmpD | - |
Lithobates sylvaticus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.08 | - |
free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | GTP | |
0.1 | - |
myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | GTP | |
0.23 | - |
myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | IMP | |
133 | - |
myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | Na+ | |
162 | - |
myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | K+ | |
254 | - |
free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | NH3 | |
702 | - |
free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | K+ | |
824 | - |
free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C | Lithobates sylvaticus | Na+ |