Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.5 extracted from

  • Sanchez-Quitian, Z.A.; Schneider, C.Z.; Ducati, R.G.; de Azevedo, W.F.; Bloch, C.; Basso, L.A.; Santos, D.S.
    Structural and functional analyses of Mycobacterium tuberculosis Rv3315c-encoded metal-dependent homotetrameric cytidine deaminase (2010), J. Struct. Biol., 169, 413-423.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.004
-
cytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis
1.059
-
2'-deoxycytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ CDA is a zinc-dependent metalloenzyme and contains one mol of Zn2+ per mol of enzyme subunit Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13938
-
4 * 13938, electrospray ionization mass spectrometry Mycobacterium tuberculosis
14000
-
4 * 14000, SDS-PAGE Mycobacterium tuberculosis
14072
-
4 * 14072, calculated from amino acid sequence Mycobacterium tuberculosis
52990
-
gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPH3
-
-
Mycobacterium tuberculosis H37Rv P9WPH3
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and butyl-Sepharose column chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.68
-
crude extract, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate Mycobacterium tuberculosis
2.35
-
crude extract, pH 7.5, at 25°C, using cytidine as substrate Mycobacterium tuberculosis
3.75
-
after 2.2fold purification, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate Mycobacterium tuberculosis
5.44
-
after 2.3fold purification, pH 7.5, at 25°C, using cytidine as substrate Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxycytidine + H2O
-
Mycobacterium tuberculosis 2'-deoxyuridine + NH3
-
?
2'-deoxycytidine + H2O
-
Mycobacterium tuberculosis H37Rv 2'-deoxyuridine + NH3
-
?
cytidine + H2O
-
Mycobacterium tuberculosis uridine + NH3
-
?
cytidine + H2O
-
Mycobacterium tuberculosis H37Rv uridine + NH3
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 13938, electrospray ionization mass spectrometry Mycobacterium tuberculosis
homotetramer 4 * 14000, SDS-PAGE Mycobacterium tuberculosis
homotetramer 4 * 14072, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
CDA
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
2'-deoxycytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis
4.8
-
cytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism CDA is a pyrimidine salvage pathway enzyme that recycles cytidine and 20-deoxycytidine for uridine and 2’-deoxyuridine synthesis, respectively Mycobacterium tuberculosis