Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.004 | - |
cytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis | |
1.059 | - |
2'-deoxycytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | CDA is a zinc-dependent metalloenzyme and contains one mol of Zn2+ per mol of enzyme subunit | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13938 | - |
4 * 13938, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
14000 | - |
4 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
14072 | - |
4 * 14072, calculated from amino acid sequence | Mycobacterium tuberculosis |
52990 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPH3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPH3 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and butyl-Sepharose column chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.68 | - |
crude extract, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate | Mycobacterium tuberculosis |
2.35 | - |
crude extract, pH 7.5, at 25°C, using cytidine as substrate | Mycobacterium tuberculosis |
3.75 | - |
after 2.2fold purification, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate | Mycobacterium tuberculosis |
5.44 | - |
after 2.3fold purification, pH 7.5, at 25°C, using cytidine as substrate | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxycytidine + H2O | - |
Mycobacterium tuberculosis | 2'-deoxyuridine + NH3 | - |
? | |
2'-deoxycytidine + H2O | - |
Mycobacterium tuberculosis H37Rv | 2'-deoxyuridine + NH3 | - |
? | |
cytidine + H2O | - |
Mycobacterium tuberculosis | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Mycobacterium tuberculosis H37Rv | uridine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 13938, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
homotetramer | 4 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
homotetramer | 4 * 14072, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
2'-deoxycytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis | |
4.8 | - |
cytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | CDA is a pyrimidine salvage pathway enzyme that recycles cytidine and 20-deoxycytidine for uridine and 2-deoxyuridine synthesis, respectively | Mycobacterium tuberculosis |