Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.5 extracted from

  • Park, Y.M.; Phi, Q.T.; Song, B.H.; Ghim, S.Y.
    Thermostability of chimeric cytidine deaminase variants produced by DNA shuffling (2009), J. Microbiol. Biotechnol., 19, 1536-1541.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Geobacillus stearothermophilus
expressed in Escherichia coli Rosetta(DE3)pLysS cells [Bacillus] caldolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
4 * 14000, SDS-PAGE Geobacillus stearothermophilus
14000
-
4 * 14000, SDS-PAGE [Bacillus] caldolyticus
16000
-
His-tagged CDA, SDS-PAGE Geobacillus stearothermophilus
16000
-
His-tagged CDA, SDS-PAGE [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus IFO12550 (T101)
-
-
-
[Bacillus] caldolyticus
-
-
-
[Bacillus] caldolyticus DSM 405 (T53)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Geobacillus stearothermophilus
Ni-NTA column chromatography [Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine + H2O
-
Geobacillus stearothermophilus uridine + NH3
-
?
cytidine + H2O
-
[Bacillus] caldolyticus uridine + NH3
-
?
cytidine + H2O
-
Geobacillus stearothermophilus IFO12550 (T101) uridine + NH3
-
?
cytidine + H2O
-
[Bacillus] caldolyticus DSM 405 (T53) uridine + NH3
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 14000, SDS-PAGE Geobacillus stearothermophilus
homotetramer 4 * 14000, SDS-PAGE [Bacillus] caldolyticus

Synonyms

Synonyms Comment Organism
CDA
-
Geobacillus stearothermophilus
CDA
-
[Bacillus] caldolyticus
cytidine/2'-deoxycytidine aminohydrolase
-
Geobacillus stearothermophilus
cytidine/2'-deoxycytidine aminohydrolase
-
[Bacillus] caldolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C Geobacillus stearothermophilus
70 90 the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C [Bacillus] caldolyticus