Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Geobacillus stearothermophilus |
expressed in Escherichia coli Rosetta(DE3)pLysS cells | [Bacillus] caldolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
4 * 14000, SDS-PAGE | Geobacillus stearothermophilus |
14000 | - |
4 * 14000, SDS-PAGE | [Bacillus] caldolyticus |
16000 | - |
His-tagged CDA, SDS-PAGE | Geobacillus stearothermophilus |
16000 | - |
His-tagged CDA, SDS-PAGE | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus IFO12550 (T101) | - |
- |
- |
[Bacillus] caldolyticus | - |
- |
- |
[Bacillus] caldolyticus DSM 405 (T53) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Geobacillus stearothermophilus |
Ni-NTA column chromatography | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine + H2O | - |
Geobacillus stearothermophilus | uridine + NH3 | - |
? | |
cytidine + H2O | - |
[Bacillus] caldolyticus | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Geobacillus stearothermophilus IFO12550 (T101) | uridine + NH3 | - |
? | |
cytidine + H2O | - |
[Bacillus] caldolyticus DSM 405 (T53) | uridine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 14000, SDS-PAGE | Geobacillus stearothermophilus |
homotetramer | 4 * 14000, SDS-PAGE | [Bacillus] caldolyticus |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Geobacillus stearothermophilus |
CDA | - |
[Bacillus] caldolyticus |
cytidine/2'-deoxycytidine aminohydrolase | - |
Geobacillus stearothermophilus |
cytidine/2'-deoxycytidine aminohydrolase | - |
[Bacillus] caldolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C | Geobacillus stearothermophilus |
70 | 90 | the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C | [Bacillus] caldolyticus |