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Literature summary for 3.5.4.5 extracted from

  • Faivre-Nitschke, E.; Grienenberger, J.M.; Gualberto, J.M.
    A prokaryotic-type cytidine deaminase from Arabidopsis thaliana gene expression and functional characterization (1999), Eur. J. Biochem., 263, 896-903.
    View publication on PubMed
Search for more literature for 3.5.4.5

Application

Application Comment Organism
medicine
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene At-cda1, located on chromosome 2 PCR amplified, cloned and sequenced, expressed in Escherichia coli BL21(D3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
blasticidin S
-
 Arabidopsis thaliana
CMP
-
 Arabidopsis thaliana
CMP
-
 Homo sapiens
CMP
-
 Saccharomyces cerevisiae
dCMP
-
 Arabidopsis thaliana
dCMP
-
 Homo sapiens
NH3
-
 Arabidopsis thaliana
NH3
-
 Escherichia coli
uridine
-
 Arabidopsis thaliana
uridine
-
 Escherichia coli
uridine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 deoxycytidine
-
 Escherichia coli
0.12
-
 deoxycytidine recombinant At-CDA1 Arabidopsis thaliana
0.22
-
 cytidine
-
 Escherichia coli
0.25
-
 cytidine
-
 Escherichia coli
0.25
-
 cytidine
-
 Saccharomyces cerevisiae
0.25
-
 cytidine
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-
cytoplasm cytosol Arabidopsis thaliana 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31600
-
 2 * 31600, SDS-PAGE Arabidopsis thaliana
32500
-
 calculated from cDNA open reading frame Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Haemophilus influenzae
-
 uridine + NH3
-
 ?
cytidine + H2O Bacillus subtilis
-
 uridine + NH3
-
 ?
cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
cytidine + H2O Homo sapiens
-
 uridine + NH3
-
 ?
cytidine + H2O Saccharomyces cerevisiae
-
 uridine + NH3
-
 ?
cytidine + H2O Arabidopsis thaliana
-
 uridine + NH3
-
 ?
cytidine + H2O Caenorhabditis elegans
-
 uridine + NH3
-
 ?
cytidine + H2O Mycoplasma pneumoniae
-
 uridine + NH3
-
 ?
cytidine + H2O Brugia pahangi
-
 uridine + NH3
-
 ?
cytidine + H2O Escherichia coli BL21(D3)
-
 uridine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Columbia
-
Bacillus subtilis
-
-
-
Brugia pahangi
-
-
-
Caenorhabditis elegans
-
-
-
Escherichia coli
-
-
-
Escherichia coli BL21(D3)
-
-
-
Haemophilus influenzae
-
-
-
Homo sapiens
-
 human
-
Mycoplasma pneumoniae
-
-
-
Saccharomyces cerevisiae
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, very stable, litle loss of enzymatic activity after 1 year Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxycytidine + H2O
-
 Homo sapiens 2',3'-dideoxyuridinel + NH3
-
 ?
2',3'-dideoxycytidine + H2O selective inhibitor of HIV reverse transcriptase Arabidopsis thaliana 2',3'-dideoxyuridinel + NH3
-
 ?
5-azacytidine + H2O
-
 Escherichia coli 5-azauridine + NH3
-
 ?
5-azacytidine + H2O
-
 Homo sapiens 5-azauridine + NH3
-
 ?
5-azacytidine + H2O
-
 Arabidopsis thaliana 5-azauridine + NH3
-
 ?
5-azacytidine + H2O
-
 Escherichia coli BL21(D3) 5-azauridine + NH3
-
 ?
cytidine + H2O
-
 Haemophilus influenzae uridine + NH3
-
 ?
cytidine + H2O
-
 Bacillus subtilis uridine + NH3
-
 ?
cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
cytidine + H2O
-
 Saccharomyces cerevisiae uridine + NH3
-
 ?
cytidine + H2O
-
 Arabidopsis thaliana uridine + NH3
-
 ?
cytidine + H2O
-
 Caenorhabditis elegans uridine + NH3
-
 ?
cytidine + H2O
-
 Mycoplasma pneumoniae uridine + NH3
-
 ?
cytidine + H2O
-
 Brugia pahangi uridine + NH3
-
 ?
cytidine + H2O
-
 Escherichia coli BL21(D3) uridine + NH3
-
 ?
cytosine arabinoside + H2O
-
 Escherichia coli uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Homo sapiens uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Arabidopsis thaliana uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Escherichia coli BL21(D3) uridine arabinoside + NH3
-
 ?
deoxycytidine + H2O
-
 Escherichia coli deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Homo sapiens deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Arabidopsis thaliana deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Escherichia coli BL21(D3) deoxyuridine + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31600, SDS-PAGE Arabidopsis thaliana
tetramer
-
 Bacillus subtilis
tetramer
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Arabidopsis thaliana