Cloned (Comment) | Organism |
---|---|
cDNA coding for AT-CDA1 amplified, subcloned into pTrc99-A, induced and expressed and overproduced in Escherichia coli SO5201 | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4,5,6-Tetrahydrouridine | - |
Arabidopsis thaliana | |
3,4,5,6-Tetrahydrouridine | - |
Escherichia coli | |
3,4,5,6-Tetrahydrouridine | - |
Mus musculus | |
3-deazauridine | - |
Arabidopsis thaliana | |
5,6-Dihydrouridine | - |
Arabidopsis thaliana | |
5,6-Dihydrouridine | - |
Escherichia coli | |
5-fluoro-2'-deoxyuridine | - |
Arabidopsis thaliana | |
5-fluorouridine | - |
Arabidopsis thaliana | |
5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine | - |
Arabidopsis thaliana | |
CMP | - |
Arabidopsis thaliana | |
thymine riboside | - |
Arabidopsis thaliana | |
thymine riboside | - |
Escherichia coli | |
uridine | - |
Arabidopsis thaliana | |
uridine | - |
Escherichia coli | |
UTP | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
cytosine arabinoside | recombinant AT-CDA | Arabidopsis thaliana | |
0.06 | - |
deoxycytidine | - |
Escherichia coli | |
0.075 | - |
deoxycytidine | recombinant AT-CDA | Arabidopsis thaliana | |
0.11 | - |
cytidine | - |
Escherichia coli | |
0.15 | - |
cytidine | recombinant AT-CDA | Arabidopsis thaliana | |
0.22 | - |
cytosine arabinoside | - |
Escherichia coli | |
0.31 | - |
5-azacytidine | recombinant AT-CDA | Arabidopsis thaliana | |
0.58 | - |
5-methylcytidine | recombinant | Arabidopsis thaliana | |
0.95 | - |
5-methylcytidine | - |
Escherichia coli | |
2.27 | - |
5-azacytidine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
4 * 15000 | Gallus gallus |
32000 | - |
2 * 32000, homodimer, SDS-PAGE | Escherichia coli |
32000 | - |
2 * 32000, homodimer, SDS-PAGE | Arabidopsis thaliana |
32560 | - |
calculated from cDNA open reading frame | Arabidopsis thaliana |
32580 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
63000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine + H2O | Gallus gallus | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Haemophilus influenzae | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Bacillus subtilis | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Mus musculus | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Ovis aries | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Arabidopsis thaliana | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Escherichia coli SO5201 | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Mus musculus BALB/c | - |
uridine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli SO5201 | - |
- |
- |
Gallus gallus | - |
chicken | - |
Haemophilus influenzae | - |
- |
- |
Mus musculus | - |
mouse | - |
Mus musculus | - |
BALB/c | - |
Mus musculus BALB/c | - |
BALB/c | - |
Ovis aries | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
74 | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-azacytidine + H2O | - |
Escherichia coli | 5-azauridine + NH3 | - |
? | |
5-azacytidine + H2O | - |
Arabidopsis thaliana | 5-azauridine + NH3 | - |
? | |
5-azacytidine + H2O | - |
Escherichia coli SO5201 | 5-azauridine + NH3 | - |
? | |
5-azadeoxycytidine + H2O | - |
Arabidopsis thaliana | 5-azadeoxyuridine + NH3 | - |
? | |
5-methylcytidine + H2O | - |
Escherichia coli | 5-methyluridine + NH3 | - |
? | |
5-methylcytidine + H2O | - |
Arabidopsis thaliana | 5-methyluridine + NH3 | - |
? | |
5-methylcytidine + H2O | - |
Escherichia coli SO5201 | 5-methyluridine + NH3 | - |
? | |
cytidine + H2O | - |
Gallus gallus | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Haemophilus influenzae | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Bacillus subtilis | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Mus musculus | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Ovis aries | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Arabidopsis thaliana | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Escherichia coli SO5201 | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Mus musculus BALB/c | uridine + NH3 | - |
? | |
cytosine arabinoside + H2O | - |
Mus musculus | uridine arabinoside + NH3 | - |
? | |
cytosine arabinoside + H2O | - |
Escherichia coli | uridine arabinoside + NH3 | - |
? | |
cytosine arabinoside + H2O | - |
Arabidopsis thaliana | uridine arabinoside + NH3 | - |
? | |
cytosine arabinoside + H2O | - |
Escherichia coli SO5201 | uridine arabinoside + NH3 | - |
? | |
cytosine arabinoside + H2O | - |
Mus musculus BALB/c | uridine arabinoside + NH3 | - |
? | |
deoxycytidine + H2O | - |
Mus musculus | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Escherichia coli | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Arabidopsis thaliana | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Escherichia coli SO5201 | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Mus musculus BALB/c | deoxyuridine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, homodimer, SDS-PAGE | Escherichia coli |
dimer | 2 * 32000, homodimer, SDS-PAGE | Arabidopsis thaliana |
tetramer | - |
Bacillus subtilis |
tetramer | 4 * 15000 | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10.5 | deoxycytidine as substrate, specific activity constant in this range, beyond these values irreversible inactivation occurs | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10.5 | deoxycytidine as substrate, beyond these extremes rapid inactivation | Arabidopsis thaliana |