Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.5 extracted from

  • Kwon, T.K.; Lee, S.Y.; Kim, J.G.; Song, B.H.; Hong, S.D.
    Purification and properties of Escherichia coli cytidine deaminase by amplification of the cdd gene (1991), Mol. Cells, 1, 281-286.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600 Escherichia coli
cdd genes cloned and characterized Salmonella enterica subsp. enterica serovar Typhimurium
cdd genes cloned and characterized Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
3,4,5,6-Tetrahydrouridine
-
Escherichia coli
5,6-Dihydrouridine competitive inhibition Escherichia coli
5-bromodeoxyuridine
-
Escherichia coli
adenosine
-
Escherichia coli
Cu2+
-
Escherichia coli
deoxyadenosine competitive inhibition Escherichia coli
deoxyguanosine
-
Escherichia coli
deoxyuridine competitive inhibition Escherichia coli
Fe2+
-
Escherichia coli
Fe3+
-
Escherichia coli
guanosine
-
Escherichia coli
Hg+
-
Escherichia coli
p-chloromercuribenzoate
-
Saccharomyces cerevisiae
thioglycolic acid 0.7 mM 88% relative activity, complete inhibition at 7 mM Escherichia coli
thymidine competitive inhibition Escherichia coli
uridine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0167
-
cytidine nonhomogenous enzyme Escherichia coli
0.089
-
cytosine deoxyriboside
-
Escherichia coli
0.21
-
deoxycytidine amplified gene Escherichia coli
0.58
-
deoxycytidine
-
Escherichia coli
2.2
-
cytidine homogenous preparation Escherichia coli
6.6
-
cytidine amplified gene, pH 7.0, 37°C Escherichia coli
8.53
-
deoxycytidine
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm inner-surface of cytoplasmic membrane Escherichia coli 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments Bacillus subtilis
33000
-
2 * 33000, SDS-PAGE Escherichia coli
34000
-
2 * 34000, SDS-PAGE Escherichia coli
54000
-
-
Escherichia coli
56000
-
calculated from Stokes radius Bacillus subtilis
56000
-
calculated from Stokes radius Escherichia coli
57000
-
gel filtration, homogenous preparation Escherichia coli
62000
-
gel filtration, native enzyme Escherichia coli
73000
-
gel filtration, nonhomogenous preparation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
uridine + NH3
-
?
cytidine + H2O Bacillus subtilis
-
uridine + NH3
-
?
cytidine + H2O Escherichia coli
-
uridine + NH3
-
?
cytidine + H2O Saccharomyces cerevisiae
-
uridine + NH3
-
?
cytidine + H2O Geobacillus stearothermophilus
-
uridine + NH3
-
?
cytidine + H2O Escherichia coli JF611
-
uridine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Escherichia coli JF611
-
-
-
Geobacillus stearothermophilus
-
-
-
Saccharomyces cerevisiae
-
bakers' yeast
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
amplified gene, 5'-aza-2'-deoxycytidine as substrate Escherichia coli
4.9
-
amplified gene, 2'-thiocytidine as substrate Escherichia coli
23.9
-
amplified gene, 5'-methylcytidine as substrate Escherichia coli
31.8
-
amplified gene, ara-cytidine as substrate Escherichia coli
43
-
amplified gene, cytidine as substrate Escherichia coli
47.9
-
amplified gene, 5'-methyl-2'-deoxycytidine as substrate Escherichia coli
68.4
-
amplified gene, 2'-fluoro-2'-deoxycytidine as substrate Escherichia coli
97.6
-
amplified gene, deoxycytidine as substrate Escherichia coli
3558
-
amplified gene Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-beta-D-arabinofuranosylcytosine + H2O
-
Escherichia coli ?
-
?
2'-deoxycytidine + H2O
-
Escherichia coli 2'-deoxyuridine + NH3
-
?
2'-deoxycytidine + H2O
-
Saccharomyces cerevisiae 2'-deoxyuridine + NH3
-
?
2'-deoxycytidine + H2O
-
Escherichia coli JF611 2'-deoxyuridine + NH3
-
?
2'-fluoro-2'-deoxycytidine + H2O
-
Escherichia coli 2'-fluoro-2'-deoxyuridine + NH3
-
?
2'-fluoro-2'-deoxycytidine + H2O
-
Escherichia coli JF611 2'-fluoro-2'-deoxyuridine + NH3
-
?
2'-thio-cytidine + H2O
-
Escherichia coli 2'-thiouridine + NH3
-
?
5'-methyl-2'-deoxycytidine + H2O
-
Escherichia coli 5'-methyl-2-deoxyuridine + NH3
-
?
5'-methyl-2'-deoxycytidine + H2O
-
Escherichia coli JF611 5'-methyl-2-deoxyuridine + NH3
-
?
5-aza-2'-deoxycytidine + H2O
-
Escherichia coli 5-aza-2'-deoxyuridine + NH3
-
?
5-methylcytidine + H2O
-
Escherichia coli 5-methyluridine + NH3
-
?
5-methylcytidine + H2O
-
Escherichia coli JF611 5-methyluridine + NH3
-
?
cytidine + H2O
-
Salmonella enterica subsp. enterica serovar Typhimurium uridine + NH3
-
?
cytidine + H2O
-
Bacillus subtilis uridine + NH3
-
?
cytidine + H2O
-
Escherichia coli uridine + NH3
-
?
cytidine + H2O
-
Saccharomyces cerevisiae uridine + NH3
-
?
cytidine + H2O
-
Geobacillus stearothermophilus uridine + NH3
-
?
cytidine + H2O
-
Escherichia coli JF611 uridine + NH3
-
?
cytosine deoxyriboside + H2O
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Escherichia coli
dimer 2 * 33000, SDS-PAGE Escherichia coli
tetramer 4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
30
-
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
relatively labile to thermal treatment, complete inactivation by incubation for 30 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
7
-
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 reasonably stable in this range Escherichia coli