Cloned (Comment) | Organism |
---|---|
cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600 | Escherichia coli |
cdd genes cloned and characterized | Salmonella enterica subsp. enterica serovar Typhimurium |
cdd genes cloned and characterized | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4,5,6-Tetrahydrouridine | - |
Escherichia coli | |
5,6-Dihydrouridine | competitive inhibition | Escherichia coli | |
5-bromodeoxyuridine | - |
Escherichia coli | |
adenosine | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
deoxyadenosine | competitive inhibition | Escherichia coli | |
deoxyguanosine | - |
Escherichia coli | |
deoxyuridine | competitive inhibition | Escherichia coli | |
Fe2+ | - |
Escherichia coli | |
Fe3+ | - |
Escherichia coli | |
guanosine | - |
Escherichia coli | |
Hg+ | - |
Escherichia coli | |
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
thioglycolic acid | 0.7 mM 88% relative activity, complete inhibition at 7 mM | Escherichia coli | |
thymidine | competitive inhibition | Escherichia coli | |
uridine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
cytidine | nonhomogenous enzyme | Escherichia coli | |
0.089 | - |
cytosine deoxyriboside | - |
Escherichia coli | |
0.21 | - |
deoxycytidine | amplified gene | Escherichia coli | |
0.58 | - |
deoxycytidine | - |
Escherichia coli | |
2.2 | - |
cytidine | homogenous preparation | Escherichia coli | |
6.6 | - |
cytidine | amplified gene, pH 7.0, 37°C | Escherichia coli | |
8.53 | - |
deoxycytidine | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | inner-surface of cytoplasmic membrane | Escherichia coli | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments | Bacillus subtilis |
33000 | - |
2 * 33000, SDS-PAGE | Escherichia coli |
34000 | - |
2 * 34000, SDS-PAGE | Escherichia coli |
54000 | - |
- |
Escherichia coli |
56000 | - |
calculated from Stokes radius | Bacillus subtilis |
56000 | - |
calculated from Stokes radius | Escherichia coli |
57000 | - |
gel filtration, homogenous preparation | Escherichia coli |
62000 | - |
gel filtration, native enzyme | Escherichia coli |
73000 | - |
gel filtration, nonhomogenous preparation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Bacillus subtilis | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Saccharomyces cerevisiae | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Geobacillus stearothermophilus | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Escherichia coli JF611 | - |
uridine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli JF611 | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Saccharomyces cerevisiae | - |
bakers' yeast | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
amplified gene, 5'-aza-2'-deoxycytidine as substrate | Escherichia coli |
4.9 | - |
amplified gene, 2'-thiocytidine as substrate | Escherichia coli |
23.9 | - |
amplified gene, 5'-methylcytidine as substrate | Escherichia coli |
31.8 | - |
amplified gene, ara-cytidine as substrate | Escherichia coli |
43 | - |
amplified gene, cytidine as substrate | Escherichia coli |
47.9 | - |
amplified gene, 5'-methyl-2'-deoxycytidine as substrate | Escherichia coli |
68.4 | - |
amplified gene, 2'-fluoro-2'-deoxycytidine as substrate | Escherichia coli |
97.6 | - |
amplified gene, deoxycytidine as substrate | Escherichia coli |
3558 | - |
amplified gene | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-beta-D-arabinofuranosylcytosine + H2O | - |
Escherichia coli | ? | - |
? | |
2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-deoxyuridine + NH3 | - |
? | |
2'-deoxycytidine + H2O | - |
Saccharomyces cerevisiae | 2'-deoxyuridine + NH3 | - |
? | |
2'-deoxycytidine + H2O | - |
Escherichia coli JF611 | 2'-deoxyuridine + NH3 | - |
? | |
2'-fluoro-2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-fluoro-2'-deoxyuridine + NH3 | - |
? | |
2'-fluoro-2'-deoxycytidine + H2O | - |
Escherichia coli JF611 | 2'-fluoro-2'-deoxyuridine + NH3 | - |
? | |
2'-thio-cytidine + H2O | - |
Escherichia coli | 2'-thiouridine + NH3 | - |
? | |
5'-methyl-2'-deoxycytidine + H2O | - |
Escherichia coli | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
5'-methyl-2'-deoxycytidine + H2O | - |
Escherichia coli JF611 | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
5-aza-2'-deoxycytidine + H2O | - |
Escherichia coli | 5-aza-2'-deoxyuridine + NH3 | - |
? | |
5-methylcytidine + H2O | - |
Escherichia coli | 5-methyluridine + NH3 | - |
? | |
5-methylcytidine + H2O | - |
Escherichia coli JF611 | 5-methyluridine + NH3 | - |
? | |
cytidine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Bacillus subtilis | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Saccharomyces cerevisiae | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Geobacillus stearothermophilus | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Escherichia coli JF611 | uridine + NH3 | - |
? | |
cytosine deoxyriboside + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Escherichia coli |
dimer | 2 * 33000, SDS-PAGE | Escherichia coli |
tetramer | 4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
30 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
relatively labile to thermal treatment, complete inactivation by incubation for 30 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
7 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | reasonably stable in this range | Escherichia coli |