Literature summary for 3.5.4.5 extracted from

  • Kidder, G.W.
    Characteristics of cytidine aminohydrolase activity in Trypanosoma cruzi and Crithidia fasciculata (1984), J. Protozool., 31, 298-300.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
thymidine
-
Crithidia fasciculata
uridine
-
Crithidia fasciculata
cytidine
-
Crithidia fasciculata
cytidine
-
Trypanosoma cruzi
5-Bromouridine
-
Crithidia fasciculata
Orotidine
-
Crithidia fasciculata

General Stability

General Stability Organism
unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions Crithidia fasciculata
unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
p-mercuribenzoate
-
Crithidia fasciculata
p-mercuribenzoate
-
Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09 0.1 deoxycytidine
-
Crithidia fasciculata
1.8 1.9 cytidine radioactive labeled substrate Trypanosoma cruzi
1.8 1.9 deoxycytidine radioactive labeled substrate Trypanosoma cruzi
2.8 3 cytidine
-
Crithidia fasciculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Trypanosoma cruzi
45000
-
gel filtration Crithidia fasciculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Crithidia fasciculata
-
uridine + NH3
-
?
cytidine + H2O Trypanosoma cruzi
-
uridine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
mosquito parasite
-
Trypanosoma cruzi
-
human pathogen, Culbertson strain, epimastigotes, ATCC 30013
-

Storage Stability

Storage Stability Organism
4°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h Trypanosoma cruzi
-20°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h Trypanosoma cruzi
4°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h Crithidia fasciculata
-20°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxycytidine + H2O
-
Crithidia fasciculata deoxyuridine + NH3
-
?
deoxycytidine + H2O
-
Trypanosoma cruzi deoxyuridine + NH3
-
?
cytidine + H2O
-
Crithidia fasciculata uridine + NH3
-
?
cytidine + H2O
-
Trypanosoma cruzi uridine + NH3
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
Crithidia fasciculata
6.5 8.5
-
Trypanosoma cruzi