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Literature summary for 3.5.4.5 extracted from
- The purification and properties of cytidine deaminase from Escherichia coli (1973), J. Biochem., 74, 797-803.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Mus musculus |  |
| EDTA | 10 mM, activity 108% | Escherichia coli | |
| iodoacetic acid | 0.10 mM, activity 105% | Escherichia coli | |
| N-ethylmaleimide | 0.10-0.20 mM, activity 114% | Escherichia coli | |
| p-chloromercuribenzoate | 0.105 mM, activity 112% | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-bromodeoxyuridine | 1.0 mM, 31% inhibition | Escherichia coli | |
| adenosine | 4.0 mM, 22% inhibition | Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| Cu2+ | - |
Mus musculus | |
| deoxyadenosine | 3.0 mM, 32% inhibition | Escherichia coli | |
| deoxyguanosine | 1.0 mM, 13% inhibition | Escherichia coli | |
| deoxyuridine | 1.0 mM, 32% inhibition | Escherichia coli | |
| dTTP | - |
Mus musculus | |
| dTTP | - |
Ovis aries | |
| dTTP | 0.400 mM 50% inhibition | Saccharomyces cerevisiae | |
| dUTP | - |
Mus musculus | |
| dUTP | - |
Ovis aries | |
| Fe2+ | - |
Escherichia coli | |
| Fe3+ | - |
Escherichia coli | |
| guanosine | 1.0 mM, 4% inhibition | Escherichia coli | |
| Hg+ | - |
Escherichia coli | |
| Hg+ | - |
Mus musculus | |
| mercaptoethanol | - |
Escherichia coli | |
| Mersalyl acid | - |
Escherichia coli | |
| Mersalyl acid | - |
Mus musculus | |
| Mersalyl acid | - |
Saccharomyces cerevisiae | |
| thymidine | 1.0 mM, 21% inhibition | Escherichia coli | |
| uracil arabinoside | 4.0 mM, 48% inhibition | Escherichia coli | |
| Urea | above 2 M, reversible inactivation | Escherichia coli | |
| uridine | 4.0 mM, 20% inhibition | Escherichia coli | |
| Zn2+ | above 10 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
5-Methyldeoxycytidine | - |
Escherichia coli | |
| 0.0853 | - |
deoxycytidine | - |
Escherichia coli | |
| 0.087 | - |
5'-fluoro-5'-deoxycytidine | - |
Escherichia coli | |
| 0.167 | - |
cytidine | - |
Escherichia coli | |
| 0.26 | - |
cytosine arabinoside | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM, activity 101% | Escherichia coli | |
| Mn2+ | - |
Mus musculus | |
| Mn2+ | 10 mM, activity 106% | Escherichia coli | |
| Zn2+ | 1 mM, activity 103% | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytidine + H2O | Mus musculus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Saccharomyces cerevisiae | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Ovis aries | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Bacillus cereus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Mus musculus BALB/c | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli Y-70-272 | - |
uridine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Escherichia coli Y-70-272 | - |
- |
- |
| Mus musculus | - |
mouse | - |
| Mus musculus | - |
BALB/c | - |
| Mus musculus BALB/c | - |
BALB/c | - |
| Ovis aries | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.53 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-fluoro-5'-deoxycytidine + H2O | - |
Escherichia coli | 5'-fluoro-5'-deoxyuridine + NH3 | - |
? | |
| 5'-fluoro-5'-deoxycytidine + H2O | - |
Escherichia coli Y-70-272 | 5'-fluoro-5'-deoxyuridine + NH3 | - |
? | |
| 5'-methyl-2'-deoxycytidine + H2O | - |
Mus musculus | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
| 5'-methyl-2'-deoxycytidine + H2O | - |
Escherichia coli | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
| 5'-methyl-2'-deoxycytidine + H2O | - |
Mus musculus BALB/c | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
| 5-methyldeoxycytidine + H2O | - |
Escherichia coli | ? | - |
? | |
| cytidine + H2O | - |
Mus musculus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Saccharomyces cerevisiae | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Ovis aries | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Bacillus cereus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Mus musculus BALB/c | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli Y-70-272 | uridine + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Mus musculus | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Escherichia coli | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Mus musculus BALB/c | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Escherichia coli Y-70-272 | uridine arabinoside + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Mus musculus | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Escherichia coli | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Mus musculus BALB/c | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Escherichia coli Y-70-272 | deoxyuridine + NH3 | - |
? | |
| additional information | cytosine and cytidine nucleosides are not deaminated by this enzyme | Escherichia coli | ? | - |
? | |
| additional information | cytosine and cytidine nucleosides are not deaminated by this enzyme | Escherichia coli Y-70-272 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mammalian deminase | - |
Mus musculus |
| mammalian deminase | - |
Ovis aries |
| yeast cytidine deaminase | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Escherichia coli |
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