Cloned (Comment) | Organism |
---|---|
wild type enzyme is expressed in Escherichia coli strain TOP10, mutant enzymes are expressed in Escherichia coli strain BL21-codon plus (DE3)-RIPL | Plasmodium vivax |
Crystallization (Comment) | Organism |
---|---|
Plasmodium vivax ADA in a complex with 5'-methylthiocoformycin is crystallized by the sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 100 mM HEPES (pH 7.5), and 0.2 M MgCl2 | Plasmodium vivax |
Protein Variants | Comment | Organism |
---|---|---|
D172A | the mutant shows no activity with 5-methylthioadenosine and is not inhibited by 5'-methylthiocoformycin | Plasmodium vivax |
D172E | the mutant shows no activity with 5-methylthioadenosine and is not inhibited by 5'-methylthiocoformycin | Plasmodium vivax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthiocoformycin | specific transition state analogue inhibitor of Plasmodium ADA | Plasmodium berghei | |
5'-methylthiocoformycin | specific transition state analogue inhibitor of Plasmodium ADA | Plasmodium cynomolgi | |
5'-methylthiocoformycin | specific transition state analogue inhibitor of Plasmodium ADA, 5'-methylthiocoformycin is a subnanomolar inhibitor of Plasmodium falciparum ADA and demonstrates more than 20000fold selectivity relative to human ADA | Plasmodium falciparum | |
5'-methylthiocoformycin | poor inhibitor of Plasmodium gallinaceum ADA | Plasmodium gallinaceum | |
5'-methylthiocoformycin | specific transition state analogue inhibitor of Plasmodium ADA | Plasmodium knowlesi | |
5'-methylthiocoformycin | specific transition state analogue inhibitor of Plasmodium ADA | Plasmodium vivax | |
coformycin | powerful picomolar inhibitor of ADA | Plasmodium berghei | |
coformycin | powerful picomolar inhibitor of ADA | Plasmodium cynomolgi | |
coformycin | powerful picomolar inhibitor of ADA | Plasmodium falciparum | |
coformycin | powerful picomolar inhibitor of ADA | Plasmodium gallinaceum | |
coformycin | powerful picomolar inhibitor of ADA | Plasmodium knowlesi | |
coformycin | powerful picomolar inhibitor of ADA | Plasmodium vivax | |
D-coformycin | powerful picomolar inhibitor of ADA | Plasmodium berghei | |
D-coformycin | powerful picomolar inhibitor of ADA | Plasmodium cynomolgi | |
D-coformycin | powerful picomolar inhibitor of ADA | Plasmodium falciparum | |
D-coformycin | powerful picomolar inhibitor of ADA | Plasmodium gallinaceum | |
D-coformycin | powerful picomolar inhibitor of ADA | Plasmodium knowlesi | |
D-coformycin | powerful picomolar inhibitor of ADA | Plasmodium vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
0.0087 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
0.0095 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.022 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
0.032 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium gallinaceum | |
0.057 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
0.06 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.083 | - |
adenosine | mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.087 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
0.088 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
0.104 | - |
adenosine | mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.115 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
0.12 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium berghei | - |
- |
- |
Plasmodium cynomolgi | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Plasmodium gallinaceum | - |
- |
- |
Plasmodium knowlesi | - |
- |
- |
Plasmodium vivax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Plasmodium knowlesi | 5'-methylthioinosine + NH3 | - |
? | |
5'-methylthioadenosine + H2O | - |
Plasmodium falciparum | 5'-methylthioinosine + NH3 | - |
? | |
5'-methylthioadenosine + H2O | - |
Plasmodium berghei | 5'-methylthioinosine + NH3 | - |
? | |
5'-methylthioadenosine + H2O | - |
Plasmodium vivax | 5'-methylthioinosine + NH3 | - |
? | |
5'-methylthioadenosine + H2O | - |
Plasmodium cynomolgi | 5'-methylthioinosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium knowlesi | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium falciparum | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium berghei | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium vivax | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium gallinaceum | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium cynomolgi | inosine + NH3 | - |
? | |
additional information | Plasmodium gallinaceum ADA does not have significant activity for 5'-methylthioadenosine | Plasmodium gallinaceum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADA | - |
Plasmodium knowlesi |
ADA | - |
Plasmodium falciparum |
ADA | - |
Plasmodium berghei |
ADA | - |
Plasmodium vivax |
ADA | - |
Plasmodium gallinaceum |
ADA | - |
Plasmodium cynomolgi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.31 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
0.35 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
0.51 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
1.8 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
1.9 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium gallinaceum | |
4.7 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
5.3 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
5.6 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
5.8 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
6.8 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
12.9 | - |
adenosine | mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
14 | - |
adenosine | mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000015 | - |
D-coformycin | mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.0000018 | - |
D-coformycin | mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.0000023 | - |
D-coformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
0.0000032 | - |
5'-methylthiocoformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
0.0000034 | - |
D-coformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
0.0000047 | - |
D-coformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium gallinaceum | |
0.000007 | - |
D-coformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
0.0000074 | - |
D-coformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.000014 | - |
5'-methylthiocoformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
0.000014 | - |
D-coformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
0.00002 | - |
5'-methylthiocoformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
0.00003 | - |
5'-methylthiocoformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
0.000048 | - |
5'-methylthiocoformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
0.029 | - |
5'-methylthiocoformycin | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium gallinaceum |
General Information | Comment | Organism |
---|---|---|
metabolism | ADA is a key enzyme in the pathway of hypoxanthine formation | Plasmodium knowlesi |
metabolism | ADA is a key enzyme in the pathway of hypoxanthine formation | Plasmodium falciparum |
metabolism | ADA is a key enzyme in the pathway of hypoxanthine formation | Plasmodium berghei |
metabolism | ADA is a key enzyme in the pathway of hypoxanthine formation | Plasmodium vivax |
metabolism | ADA is a key enzyme in the pathway of hypoxanthine formation | Plasmodium gallinaceum |
metabolism | ADA is a key enzyme in the pathway of hypoxanthine formation | Plasmodium cynomolgi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
23 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
30 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
36 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
50 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
57 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium knowlesi | |
59 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium gallinaceum | |
61 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium cynomolgi | |
64 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium falciparum | |
79 | - |
5'-methylthioadenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
82 | - |
adenosine | wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium berghei | |
130 | - |
adenosine | mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax | |
150 | - |
adenosine | mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication | Plasmodium vivax |