Protein Variants | Comment | Organism |
---|---|---|
additional information | effects of sodium dodecyl sulfate, dodecyltrimethylammonium bromide, sodium chloride, sodium sulfate, methanol and ethanol, on the structure and activity of adenosine deaminase are investigated by UV-Vis, circular dichroism spectrophotometry and molecular dynamics studies. Relative activity, experimental and computational helix content, total accessible surface area and exposed charged surface area are obtained. The relative activity of adenosine deaminase in the absence and the presence of denaturants is compared with structural results. It is shown that an increase in the surface area and a decrease in the amount of helicity are associated with a decrease in the activity of ADA | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dodecyltrimethylammonium bromide | at low concentrations (approximately 0.1 mM), enzyme activity decreases, it increases at a slightly higher concentration (about 0.2 mM), and finally decreases again (about 0.3 mM), CD spectra of adenosine deaminase and DTAB, determination of helix content, MD calculations | Bos taurus | |
ethanol | CD spectra of adenosine deaminase and EtOH, determination of helix content, MD calculations | Bos taurus | |
methanol | MD calculations, discussion of effects | Bos taurus | |
additional information | the trend of relative activity is directly proportional to helicity and inversely proportional to accessible surface area | Bos taurus | |
Na2SO4 | CD spectra of adenosine deaminase and Na2SO4, determination of helix content, MD calculations, discussion of effects | Bos taurus | |
NaCl | MD calculations, discussion of effects | Bos taurus | |
Sodium dodecyl sulfate | at low concentrations (approximately 0.1 mM) enzyme activity decreases, it increases at a slightly higher concentration (about 0.4 mM), and finally decreases again (about 0.55 mM), CD spectra of adenosine deaminase and SDS, determination of helix content, MD calculations | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P56658 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestinal mucosa | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Bos taurus | inosine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADA | - |
Bos taurus |
adenosine deaminase | - |
Bos taurus |