Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 44% inhibition at 5 mM | Fasciola gigantica | |
Ca2+ | 69% inhibition at 5 mM | Fasciola gigantica | |
Cu2+ | 81% inhibition at 5 mM | Fasciola gigantica | |
Hg2+ | 88% inhibition at 5 mM | Fasciola gigantica | |
Mn2+ | 85% inhibition at 5 mM | Fasciola gigantica | |
Ni2+ | 61% inhibition at 5 mM | Fasciola gigantica | |
Zn2+ | 65% inhibition at 5 mM | Fasciola gigantica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
adenosine | pH 7.2, 37°C, ADAII | Fasciola gigantica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Fasciola gigantica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, ADAII, SDS-PAGE | Fasciola gigantica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | Fasciola gigantica | - |
inosine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola gigantica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native ADAI 12.3fold by acetone precipitation and ion exchange chromatography, native ADAII 176.6fold by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity | Fasciola gigantica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
75.7 U/g tissue, ADAII | Fasciola gigantica |
4.4 | - |
partially purified ADAI | Fasciola gigantica |
62.7 | - |
purified ADAII | Fasciola gigantica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + H2O | ADAII shows 88% of the activity with adenosine | Fasciola gigantica | 2'-deoxyinosine + NH3 | - |
? | |
2'-deoxyguanosine + H2O | ADAII shows 41% of the activity with adenosine | Fasciola gigantica | 2'-deoxyxanthosine + NH3 | - |
? | |
adenine + H2O | ADAII shows 70% of the activity with adenosine | Fasciola gigantica | hypoxanthine + NH3 | - |
? | |
adenosine + H2O | - |
Fasciola gigantica | inosine + NH3 | - |
? | |
adenosine + H2O | preferred substrate | Fasciola gigantica | inosine + NH3 | - |
? | |
guanine + H2O | ADAII shows 47% of the activity with adenosine | Fasciola gigantica | xanthine + NH3 | - |
? | |
guanosine + H2O | ADAII shows 75% of the activity with adenosine | Fasciola gigantica | xanthosine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, ADAII, SDS-PAGE | Fasciola gigantica |
Synonyms | Comment | Organism |
---|---|---|
ADA | - |
Fasciola gigantica |
ADAI | - |
Fasciola gigantica |
ADAII | - |
Fasciola gigantica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
ADAII | Fasciola gigantica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | activity range of ADAII | Fasciola gigantica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
ADAII, stable up to | Fasciola gigantica |
90 | - |
loss of 95% activity, ADAII | Fasciola gigantica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
ADAII | Fasciola gigantica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | ADAII shows 7% of maximal activity at pH 5.5 and 86% at pH 9.0 | Fasciola gigantica |