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Literature summary for 3.5.4.4 extracted from

  • Ali, E.M.
    Fasciola gigantica: purification and characterization of adenosine deaminase (2008), Exp. Parasitol., 119, 285-290.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 44% inhibition at 5 mM Fasciola gigantica
Ca2+ 69% inhibition at 5 mM Fasciola gigantica
Cu2+ 81% inhibition at 5 mM Fasciola gigantica
Hg2+ 88% inhibition at 5 mM Fasciola gigantica
Mn2+ 85% inhibition at 5 mM Fasciola gigantica
Ni2+ 61% inhibition at 5 mM Fasciola gigantica
Zn2+ 65% inhibition at 5 mM Fasciola gigantica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
adenosine pH 7.2, 37°C, ADAII Fasciola gigantica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Fasciola gigantica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, ADAII, SDS-PAGE Fasciola gigantica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine + H2O Fasciola gigantica
-
inosine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Fasciola gigantica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native ADAI 12.3fold by acetone precipitation and ion exchange chromatography, native ADAII 176.6fold by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity Fasciola gigantica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
75.7 U/g tissue, ADAII Fasciola gigantica
4.4
-
partially purified ADAI Fasciola gigantica
62.7
-
purified ADAII Fasciola gigantica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O ADAII shows 88% of the activity with adenosine Fasciola gigantica 2'-deoxyinosine + NH3
-
?
2'-deoxyguanosine + H2O ADAII shows 41% of the activity with adenosine Fasciola gigantica 2'-deoxyxanthosine + NH3
-
?
adenine + H2O ADAII shows 70% of the activity with adenosine Fasciola gigantica hypoxanthine + NH3
-
?
adenosine + H2O
-
Fasciola gigantica inosine + NH3
-
?
adenosine + H2O preferred substrate Fasciola gigantica inosine + NH3
-
?
guanine + H2O ADAII shows 47% of the activity with adenosine Fasciola gigantica xanthine + NH3
-
?
guanosine + H2O ADAII shows 75% of the activity with adenosine Fasciola gigantica xanthosine + NH3
-
?

Subunits

Subunits Comment Organism
? x * 29000, ADAII, SDS-PAGE Fasciola gigantica

Synonyms

Synonyms Comment Organism
ADA
-
Fasciola gigantica
ADAI
-
Fasciola gigantica
ADAII
-
Fasciola gigantica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
ADAII Fasciola gigantica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 activity range of ADAII Fasciola gigantica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
ADAII, stable up to Fasciola gigantica
90
-
loss of 95% activity, ADAII Fasciola gigantica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
ADAII Fasciola gigantica

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 ADAII shows 7% of maximal activity at pH 5.5 and 86% at pH 9.0 Fasciola gigantica