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Literature summary for 3.5.4.4 extracted from
- Human plasma adenosine deaminase 2 is secreted by activated monocytes (2006), Biol. Chem., 387, 319-321.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxyformycin | 50% inhibition of isozyme ADA2 at 0.09 mM | Homo sapiens |  |
| erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. EHNA, specific for isozyme ADA1, no inhibition of isozyme ADA2 | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | isozymes ADA1 and ADA2 are kinetically distinct | Homo sapiens | |
| 2.15 | - |
adenosine | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | isoyzme ADA2 is secreted by activated monocytes to blood plasma | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
2 * 67000, SDS-PAGE | Homo sapiens |
| 110000 | - |
isozyme ADA2, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyadenosine + H2O | Homo sapiens | the enzyme plays an important role in the immune system | 2'-deoxyinosine + NH3 | - |
? | |
| adenosine + H2O | Homo sapiens | the enzyme plays an important role in the immune system | inosine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozymes ADA1 and ADA2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozyme ADA2 400fold from plasma by ammonium sulfate fractionation, anion exchange chromatography, adsorption chromatography, and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| B-cell | isozyme ADA1 | Homo sapiens | - |
| blood plasma | isozyme ADA2 | Homo sapiens | - |
| monocyte | of peripheral blood | Homo sapiens | - |
| additional information | distribution of isozymes | Homo sapiens | - |
| T-lymphocyte | isoyzme ADA1 | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.2 | - |
purified isozyme ADA2 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyadenosine + H2O | - |
Homo sapiens | 2'-deoxyinosine + NH3 | - |
? | |
| 2'-deoxyadenosine + H2O | the enzyme plays an important role in the immune system | Homo sapiens | 2'-deoxyinosine + NH3 | - |
? | |
| adenosine + H2O | - |
Homo sapiens | inosine + NH3 | - |
? | |
| adenosine + H2O | the enzyme plays an important role in the immune system | Homo sapiens | inosine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 67000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADA2 | - |
Homo sapiens |
| adenosine deaminase 2 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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