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Literature summary for 3.5.4.4 extracted from

  • Iwaki-Egawa, S.; Watanabe, Y.
    Characterization and purification of adenosine deaminase 1 from human and chicken liver (2002), Comp. Biochem. Physiol. B, 133, 173-182.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxycoformycin 0.0015 nM, 50% inhibition Gallus gallus
2'-deoxycoformycin 0.0004 nM, 50% inhibition Homo sapiens
erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride 0.4 nM, 50% inhibition Gallus gallus
erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride 0.45 nM, 50% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000023
-
adenosine
-
Gallus gallus
0.000046
-
adenosine
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
a second band of 300000 Da is detected for the complex of adenosine deaminase 1 with dipeptidyl peptidase IV, non-denaturing PAGE Homo sapiens
42000
-
1 * 42000, enzyme exists as monomer, not in complex with dipeptidyl peptidase IV, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
-
Gallus gallus
28.49
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-deoxyadenosine + H2O 1.7% of the activity with adenosine Gallus gallus 2',3'-deoxyinosine + NH3
-
?
2'-deoxyadenosine + H2O 79.2% of the activity with adenosine Gallus gallus 2'-deoxyinosine + NH3
-
?
2'-deoxyadenosine + H2O 82.8% of the activity with adenosine Homo sapiens 2'-deoxyinosine + NH3
-
?
3'-deoxyadenosine + H2O 2.4% of the activity with adenosine Gallus gallus 3'-deoxyinosine + NH3
-
?
adenosine + H2O
-
Gallus gallus inosine + NH3
-
?
adenosine + H2O
-
Homo sapiens inosine + NH3
-
?
additional information activity with 3'-deoxyadenosine, 2',3'-deoxyadenosine and 2'-O-methyladenosine is less than 0.5% of the activity with adenine Homo sapiens ?
-
?
additional information no activity with 2'-O-methyladenosine Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, enzyme exists as monomer, not in complex with dipeptidyl peptidase IV, SDS-PAGE Gallus gallus
monomer 1 * 42000, in human liver the adenosine deaminase 1 mainly exists as a complex with dipeptidyl peptidase IV. The molar ratio of adenosine deaminase 1 to dipeptidyl peptidase IV appears to be 2:1, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ADA1
-
Gallus gallus
ADA1
-
Homo sapiens
adenosine deaminase 1
-
Gallus gallus
adenosine deaminase 1
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Gallus gallus
7.5 8
-
Homo sapiens