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Literature summary for 3.5.4.4 extracted from

  • Nygaard, P.
    Adenosine deaminase from Escherichia coli (1978), Methods Enzymol., 51, 508-512.
    View publication on PubMed
Search for more literature for 3.5.4.4

Inhibitors

Inhibitors Comment Organism Structure
coformycin
-
 Escherichia coli
p-chloromercuribenzoate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 2'-deoxyadenosine
-
 Escherichia coli
0.075
-
 adenosine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
277
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
4°C, Tris-HCl, pH 8.2, 20% ethylene glycol, 2 mM CaCl2, 50µM EDTA, several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxyadenosine + H2O
-
 Escherichia coli 2',3'-dideoxyinosine + NH3
-
 ?
2'-deoxyadenosine + H2O
-
 Escherichia coli 2'-deoxyinosine + NH3
-
 ?
3'-deoxyadenosine + H2O
-
 Escherichia coli 3'-deoxyinosine + NH3
-
 ?
6-methylaminopurine ribonucleoside + H2O
-
 Escherichia coli inosine + methylamine
-
 ?
adenine + H2O
-
 Escherichia coli hypoxanthine + NH3
-
 ?
adenine arabinoside + H2O
-
 Escherichia coli inosine arabinoside + NH3
-
 ?
adenosine + H2O
-
 Escherichia coli inosine + NH3
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 8.5
-
 Escherichia coli