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Literature summary for 3.5.4.4 extracted from

  • Akedo, H.; Nishihara, H.; Shinkai, K.; Komatsu, K.; Ishikawa, S.
    Multiple forms of human adenosine deaminase. 1. Purification and characterization of two molecular species (1972), Biochim. Biophys. Acta, 276, 257-271.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-Aminopurine
-
Homo sapiens
adenine
-
Homo sapiens
Cu2+
-
Homo sapiens
guanosine
-
Homo sapiens
Hg2+
-
Homo sapiens
Inosine
-
Homo sapiens
p-chloromercuryphenylsulfonate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
adenosine El Homo sapiens
0.074
-
adenosine Es Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
Es, gel filtration Homo sapiens
230000
-
El, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
normal B-cells and T-cell lines MOLT-4
-
Homo sapiens
-
RPMI-8402
-
Homo sapiens
-
CCRF-CEM
-
Homo sapiens
-
larger enzyme: El, smaller enzyme: Es
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
lung
-
Homo sapiens
-
stomach
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
El Homo sapiens
17.4
-
Es Homo sapiens

Storage Stability

Storage Stability Organism
0°C, phosphate buffer, pH 7.0, 2-mercaptoethanol, 1 month, enzyme EI Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O
-
Homo sapiens 2'-deoxyinosine + NH3
-
?
adenosine + H2O
-
Homo sapiens inosine + NH3
-
?

Subunits

Subunits Comment Organism
polymer
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens