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Literature summary for 3.5.4.38 extracted from

  • Bae, S.J.; Park, B.G.; Kim, B.G.; Hahn, J.S.
    Multiplex gene disruption by targeted base editing of Yarrowia lipolytica genome using cytidine deaminase combined with the CRISPR/Cas9 system (2019), Biotechnol. J., 2019, e1900238 .
    View publication on PubMed

Application

Application Comment Organism
molecular biology a target-AID base editor, designed to recruit cytidine deaminase (CDA) to the target DNA locus via the CRISPR/Cas9 system, can directly induce C to T mutation without double-strand breaks and donor DNA. This system is adopted in Yarrowia lipolytica for multiplex gene disruption. Target-specific gRNA(s) and a fusion protein consisting of a nickase Cas9, CDA1, and uracil DNA glycosylase inhibitor are expressed from a single plasmid to disrupt target genes by introducing a stop codon via C to T mutation within the mutational window. Using this Target-AID system, single gene disruption and simultaneous double gene disruption are achieved with the efficiencies up to 94% and 31%, respectively Petromyzon marinus

Organism

Organism UniProt Comment Textmining
Petromyzon marinus
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Synonyms

Synonyms Comment Organism
activation-induced cytidine deaminase
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Petromyzon marinus
CDA1
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Petromyzon marinus