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Literature summary for 3.5.4.38 extracted from

  • Dickerson, S.K.; Market, E.; Besmer, E.; Papavasiliou, F.N.
    AID mediates hypermutation by deaminating single stranded DNA (2003), J. Exp. Med., 197, 1291-1296.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E58Q/C87A/C90A the mutant enzyme does not bind to single-stranded DNA at all, though it retains some binding to RNA Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine in single-stranded DNA + H2O Homo sapiens AID mediates hypermutation by deaminating single stranded DNA. In vivo, single stranded DNA may arise transiently during transcription uracil in single-stranded DNA + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9GZX7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine in single-stranded DNA + H2O AID mediates hypermutation by deaminating single stranded DNA. In vivo, single stranded DNA may arise transiently during transcription Homo sapiens uracil in single-stranded DNA + NH3
-
?
cytosine in single-stranded DNA + H2O the purified, tetrameric enzyme can deaminate cytidine residues in single-stranded DNA, but not in RNA. It deaminates only single-stranded DNA. Neither cytidine nor CTP (or dCTP) are good substrates for the enzyme in vitro Homo sapiens uracil in single-stranded DNA + NH3
-
?

Subunits

Subunits Comment Organism
tetramer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
activation-induced deaminase
-
Homo sapiens
AID
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 37 the enzyme works with varying efficiencies from room temperature to 37°C Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
30 min, protein is active Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.6 9 wide range of pH Homo sapiens

General Information

General Information Comment Organism
physiological function AID mediates hypermutation by deaminating single stranded DNA. In vivo, single stranded DNA may arise transiently during transcription Homo sapiens