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Literature summary for 3.5.4.30 extracted from

  • Siggaard, J.H.; Johansson, E.; Vognsen, T.; Helt, S.S.; Harris, P.; Larsen, S.; Willemoes, M.
    Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: a structural and pre-steady state kinetic analysis (2009), Arch. Biochem. Biophys., 490, 42-49.
    View publication on PubMed
Search for more literature for 3.5.4.30

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme E145A in complex with the substrate analogue alpha,beta-imido-dUTP and mutant enzyme E145Q in complex with diphosphate, hanging drop vapor diffusion method, using 0.2 M potassium citrate (E135Q) or 0.2 M potassium hydrogen phosphate (E145A) and 20% (w/v) PEG3350 Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
E145A the mutant enzyme is defect in the deaminase reaction Methanocaldococcus jannaschii
E145Q the mutant enzyme is defect in the deaminase reaction Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
alpha,beta-imido-dUTP
-
 Methanocaldococcus jannaschii
dTTP
-
 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCTP + H2O Methanocaldococcus jannaschii
-
 dUMP + diphosphate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57872
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + H2O
-
 Methanocaldococcus jannaschii dUMP + diphosphate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
dCTP deaminase-dUTPase bifunctional enzyme Methanocaldococcus jannaschii

General Information

General Information Comment Organism
metabolism the bifunctional dCTP deaminase-dUTPase coordinates the deamination and triphosphate cleavage reactions Methanocaldococcus jannaschii