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Literature summary for 3.5.4.30 extracted from
- The Methanococcus jannaschii dCTP deaminase is a bifunctional deaminase and diphosphatase (2003), J. Biol. Chem., 278, 11100-11106.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E145Q | no deaminase activity | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | 42% inhibition at 1 mM | Methanocaldococcus jannaschii |  |
| dUTP | 80% inhibition at 1 mM | Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17.6 | - |
dCTP | 60°C, pH 7.5 | Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, optimal concentration: 2-5 mM | Methanocaldococcus jannaschii | |
| additional information | requirement for divalent cation | Methanocaldococcus jannaschii | |
| NaCl | maximum activity at 0.15 or 2 M | Methanocaldococcus jannaschii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCTP + H2O | Methanocaldococcus jannaschii | - |
dUMP + diphosphate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | Q57872 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCTP + H2O | - |
Methanocaldococcus jannaschii | dUMP + diphosphate + NH3 | - |
? | |
| additional information | enzyme has also dUTP diphosphatase activity, dCTP deamination and dUTP diphosphatase activities occur independently at the same active site | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | no substrates: dCMP, CTP, CDP, CMP, cytosine, deoxycytidine, ATP, dTTP, UTP and GTP | Methanocaldococcus jannaschii | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
thermostable, loss of 30% activity after 10 min | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Methanocaldococcus jannaschii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 50% activity at pH 6 and pH 9 | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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