Cloned (Comment) | Organism |
---|---|
expressed in Arxula adeninivorans strain G1212 | Blastobotrys adeninivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by EDTA, 1,10-phenanthroline, dithiothreitol, L-cysteine, and phenylmethylsulfonyl fluoride | Blastobotrys adeninivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
guanine | recombinant enzyme, at pH 6.0 and 37°C | Blastobotrys adeninivorans | |
0.056 | - |
guanine | native enzyme, at pH 6.0 and 37°C | Blastobotrys adeninivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Blastobotrys adeninivorans | 5737 | - |
vacuole | - |
Blastobotrys adeninivorans | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | at 1 mM Cu2+, the native enzyme shows about 80% inhibition and the recombinant enzyme almost complete (99%) inhibition | Blastobotrys adeninivorans | |
Hg2+ | complete inhibition at 1 mM | Blastobotrys adeninivorans | |
additional information | not influenced by Ca2+, Fe3+, K+, Mg2+, Ni2+, and Zn2+ | Blastobotrys adeninivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, calculated from amino acid sequence | Blastobotrys adeninivorans |
55000 | - |
2 * 55000, SDS-PAGE and MALDI-TOF mass spectrometry | Blastobotrys adeninivorans |
100000 | 110000 | recombinant and native enzyme, gel filtration | Blastobotrys adeninivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Blastobotrys adeninivorans | 100% activity | xanthine + NH3 | - |
r | |
guanine + H2O | Blastobotrys adeninivorans LS3 | 100% activity | xanthine + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobotrys adeninivorans | - |
- |
- |
Blastobotrys adeninivorans LS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration | Blastobotrys adeninivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
crude extract, at pH 6.0 and 37°C | Blastobotrys adeninivorans |
4.7 | - |
after 51.5fold purification, at pH 6.0 and 37°C | Blastobotrys adeninivorans |
Storage Stability | Organism |
---|---|
-20°C, 28 days, 123% activity | Blastobotrys adeninivorans |
-20°C, with 20% (v/v) glycine, 28 days, 65% residual activity | Blastobotrys adeninivorans |
22°C, 28 days, 34% residual activity | Blastobotrys adeninivorans |
4°C, 28 days, 44% residual activity | Blastobotrys adeninivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-diaminopurine + H2O | the native enzyme shows 107% activity and the recombinant enzyme 98% activity compared to guanine | Blastobotrys adeninivorans | ? | - |
? | |
2-amino-6-chloropurine + H2O | the native enzyme shows 93% activity and the recombinant enzyme 88% activity compared to guanine | Blastobotrys adeninivorans | 6-chloro-9H-purin-2-ol + NH3 | - |
? | |
8-azaguanine + H2O | the native enzyme shows 99% activity and the recombinant enzyme 95% activity compared to guanine | Blastobotrys adeninivorans | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | the native enzyme shows 99% activity and the recombinant enzyme 95% activity compared to guanine | Blastobotrys adeninivorans LS3 | 8-azaxanthine + NH3 | - |
? | |
8-azaxanthine + H2O | the native enzyme shows 95% activity and the recombinant enzyme 88% activity compared to guanine | Blastobotrys adeninivorans | ? | - |
? | |
adenine + H2O | the native enzyme shows 102% activity and the recombinant enzyme 85% activity compared to guanine | Blastobotrys adeninivorans | allopurinol + NH3 | - |
? | |
adenine + H2O | the native enzyme shows 102% activity and the recombinant enzyme 85% activity compared to guanine | Blastobotrys adeninivorans LS3 | allopurinol + NH3 | - |
? | |
adenosine + H2O | the native enzyme shows 98% activity and the recombinant enzyme 91% activity compared to guanine | Blastobotrys adeninivorans | inosine + NH3 | - |
? | |
adenosine + H2O | the native enzyme shows 98% activity and the recombinant enzyme 91% activity compared to guanine | Blastobotrys adeninivorans LS3 | inosine + NH3 | - |
? | |
guanine + H2O | 100% activity | Blastobotrys adeninivorans | xanthine + NH3 | - |
r | |
guanine + H2O | 100% activity | Blastobotrys adeninivorans LS3 | xanthine + NH3 | - |
r | |
guanosine + H2O | the native enzyme shows 48% activity and the recombinant enzyme 70% activity compared to guanine | Blastobotrys adeninivorans | ? | - |
? | |
hypoxanthine + H2O | the native enzyme shows 100% activity and the recombinant enzyme 87% activity compared to guanine | Blastobotrys adeninivorans | ? | - |
? | |
uric acid + H2O | the native enzyme shows 89% activity and the recombinant enzyme 86% activity compared to guanine | Blastobotrys adeninivorans | ? | - |
? | |
uric acid + H2O | the native enzyme shows 89% activity and the recombinant enzyme 86% activity compared to guanine | Blastobotrys adeninivorans LS3 | ? | - |
? | |
xanthine + H2O | the native enzyme shows 01% activity and the recombinant enzyme 87% activity compared to guanine | Blastobotrys adeninivorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 53000, calculated from amino acid sequence | Blastobotrys adeninivorans |
homodimer | 2 * 55000, SDS-PAGE and MALDI-TOF mass spectrometry | Blastobotrys adeninivorans |
Synonyms | Comment | Organism |
---|---|---|
Agdap | - |
Blastobotrys adeninivorans |
GDA | - |
Blastobotrys adeninivorans |
Guanine aminohydrolase | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Blastobotrys adeninivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the native enzyme remains stable for 1 h at up to 30°C | Blastobotrys adeninivorans |
60 | - |
the recombinant enzyme remains stable for 1 h at up to 60°C | Blastobotrys adeninivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
guanine | recombinant enzyme, at pH 6.0 and 37°C | Blastobotrys adeninivorans | |
3.9 | - |
guanine | native enzyme, at pH 6.0 and 37°C | Blastobotrys adeninivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
the native enzyme shows optimal pH values of 6.5 and 8.0 while the recombinant enzyme has a single pH optimum at pH 6.5 | Blastobotrys adeninivorans |
8 | - |
the native enzyme shows optimal pH values of 6.5 and 8.0 | Blastobotrys adeninivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | native and recombinant enzymes are stable during incubation for 1 h at 37°C with pH in the range of 6.5-8.0 | Blastobotrys adeninivorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Blastobotrys adeninivorans | calculated from amino acid sequence | - |
5.7 |
Organism | Comment | Expression |
---|---|---|
Blastobotrys adeninivorans | immediately after the shift of cells into media with purine as nitrogen source, the enzyme transcript level increases and reached maximum level at 2 h with adenine and 4 h with hypoxanthine, after which transcription fell to the basal level | up |