Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in COS-7 cells, subcloning in Escherichia coli strain DH5alpha | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D330A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Rattus norvegicus |
H240A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Rattus norvegicus |
H279A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Rattus norvegicus |
H71A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Rattus norvegicus |
H82A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Rattus norvegicus |
H84A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Rattus norvegicus |
I83A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | essential for activity, binding structure, computational homology modeling shows that several histidines and aspartic acid residues are responsible for zinc binding, e.g. His82, His84, His240, His279, and Asp330, overview | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Rattus norvegicus | the enzyme plays a central role in neuronal development, and an active role in regulating dendrite branching in hippocampal neurons, cypin-promoted increases in dendrite number are dependent on its zinc-dependent guanine deaminase activity, overview | xanthine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
hippocampus | - |
Rattus norvegicus | - |
neuron | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
guanine + H2O | the enzyme plays a central role in neuronal development, and an active role in regulating dendrite branching in hippocampal neurons, cypin-promoted increases in dendrite number are dependent on its zinc-dependent guanine deaminase activity, overview | Rattus norvegicus | xanthine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | computational homology modeling techniques to construct a three dimensional structural model of cypin, overview | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
cypin | - |
Rattus norvegicus |
cytosolic PSD-95 interactor | - |
Rattus norvegicus |
More | cypin is a member of the metal-dependent aminohydrolase family | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |