Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | 0.4 mM, residual enzyme activity 112% | Rattus norvegicus | |
ATP | 0.4 mM, residual enzyme activity 104% | Rattus norvegicus | |
uric acid | 0.4 mM, residual enzyme activity 105% | Rattus norvegicus | |
xanthine | 0.4 mM, residual enzyme activity 110% | Rattus norvegicus | |
XMP | 0.4 mM, residual enzyme activity 113% | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
5-aminoimidazole-4-carboxamide ribonucleoside | AICAR | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
guanine | - |
Sus sp. | |
0.17 | - |
guanine | - |
Rattus norvegicus | |
0.67 | - |
8-Azaguanine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Sus sp. |
52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Rattus norvegicus |
105000 | - |
gel filtration | Rattus norvegicus |
108000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Sus sp. | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Rattus norvegicus Wistar | - |
xanthine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Rattus norvegicus | - |
Wistar strain | - |
Rattus norvegicus Wistar | - |
Wistar strain | - |
Sus sp. | - |
pig | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Sus sp. | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.7 | - |
purified brain enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, 10 mM phosphate buffer, pH 7.0, 0.1 mM dithiothreitol, can be stored for more than 2 weeks without appreciable loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-azaguanine + H2O | - |
Rattus norvegicus | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Rattus norvegicus Wistar | 8-azaxanthine + NH3 | - |
? | |
guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Sus sp. | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Rattus norvegicus Wistar | xanthine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Sus sp. |
dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.4 | - |
Rattus norvegicus |