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Literature summary for 3.5.4.3 extracted from

  • Negishi, O.; Ozawa, T.; Imagawa, H.
    Guanosine deaminase and guanine deaminase from tea leaves (1994), Biosci. Biotechnol. Biochem., 58, 1277-1281.
No PubMed abstract available
Search for more literature for 3.5.4.3

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, relative activity 104% Camellia sinensis
ICH2COOH 1mM, relative activity 103% Camellia sinensis
NaF 1 mM, relative activity 102% Camellia sinensis

Inhibitors

Inhibitors Comment Organism Structure
Cd(CH3COO)2 relative activity 7.4% Camellia sinensis
CuSO4 relative activity 25.8% Camellia sinensis
PCMB relative activity 34.7% Camellia sinensis
Zn2+ 1 mM ZnSO4, complete inhibition Camellia sinensis
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0417
-
 guanine
-
 Camellia sinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 1 mM AlCl3, relative activity 102% Camellia sinensis
Ca2+
-
 Rattus norvegicus
Ca2+ 1 mM CaCl2, relative activity 101% Camellia sinensis
Mg2+
-
 Rattus norvegicus
Mg2+ 1 mM MgCl2, relative activity 102% Camellia sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 gel filtration Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + H2O Rattus norvegicus
-
 xanthine + NH3
-
 ?
guanine + H2O Nicotiana tabacum
-
 xanthine + NH3
-
 ?
guanine + H2O Camellia sinensis
-
 xanthine + NH3
-
 ?
guanine + H2O Clostridium sp.
-
 xanthine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis
-
 tea, L. O.Kuntze cv. Yabukita
-
Clostridium sp.
-
 C. acidiurici
-
Nicotiana tabacum
-
-
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Camellia sinensis
-
 Clostridium sp.

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
leaf
-
 Nicotiana tabacum
-
leaf
-
 Camellia sinensis
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.8
-
-
 Camellia sinensis

Storage Stability

Storage Stability Organism
-20°C stable for 2 weeks with only a few percent loss Camellia sinensis
4°C stable for 2 weeks with only a few percent loss Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + H2O
-
 Camellia sinensis allopurinol + NH3
-
 ?
cytidine + H2O
-
 Camellia sinensis uridine + NH3
-
 ?
cytosine + H2O
-
 Camellia sinensis 6-hydroxy-1H-pyrimidin-2-one + NH3
-
 ?
guanine + H2O
-
 Rattus norvegicus xanthine + NH3
-
 ?
guanine + H2O
-
 Nicotiana tabacum xanthine + NH3
-
 ?
guanine + H2O
-
 Camellia sinensis xanthine + NH3
-
 ?
guanine + H2O
-
 Clostridium sp. xanthine + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Camellia sinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
 Rattus norvegicus
50
-
 activity rapidly decreases above Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 double peaks of optimum activity, second optimum at pH 8.5 Rattus norvegicus
7 7.5 double peaks of optimum activity, second optimum at pH 8.5 Camellia sinensis
8.5
-
 double peaks of optimum activity, second optimum between pH 7 and pH 7.5 Rattus norvegicus
8.5
-
 double peaks of optimum activity, second optimum between pH 7 and pH 7.5 Camellia sinensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9
-
 Camellia sinensis