General Stability | Organism |
---|---|
addition of K2HPO4 or other salts prevents inactivation, relatively low concentrations of the salt, below 0.1 mM, are required for the stabilization, potassium salts being more effective than ammonium and sodium salts, at concentrations above 0.5 M, which are required for maximal activity the thermostability decreases again | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of salts on Km | Methanopyrus kandleri | |
0.1 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | 1 M K2HPO4 | Methanopyrus kandleri | |
0.14 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | 0.6 M K2HPO4 | Methanopyrus kandleri | |
0.2 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | 0.3 M K2HPO4 | Methanopyrus kandleri | |
2 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 1.5 M, stimulates | Methanopyrus kandleri | |
ammonium sulfate | 1.5 M, maximal 100fold stimulation | Methanopyrus kandleri | |
K2HPO4 | maximal activation by 1.5 M K2HPO4, 1 M Na2HPO4, or 1 M Na2SO4 | Methanopyrus kandleri | |
K2HPO4 | 200fold increase of activity at 1.5 M | Methanopyrus kandleri | |
K2SO4 | 0.8 M stimulates | Methanopyrus kandleri | |
K2SO4 | 0.8 M, maximal 100fold stimulation | Methanopyrus kandleri | |
KCl | 2.5 M stimulates | Methanopyrus kandleri | |
KCl | 2.5 M, maximal 100fold stimulation | Methanopyrus kandleri | |
Na2HPO4 | maximal activation by 1.5 M K2HPO4, 1 M Na2HPO4 or 1 M Na2SO4 | Methanopyrus kandleri | |
Na2SO4 | maximal activation by 1.5 M K2HPO4, 1 M Na2HPO4 or 1 M Na2SO4 | Methanopyrus kandleri | |
NaCl | 2.0 M, stimulates | Methanopyrus kandleri | |
NaCl | 2 M, maximal 100fold stimulation | Methanopyrus kandleri | |
NH4Cl | optimal concentration is 0.3 M | Methanopyrus kandleri | |
Sodium phosphate | 1 M, 200fold stimulation | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
1 * 41500, SDS-PAGE | Methanopyrus kandleri |
42000 | - |
non-denaturing PAGE | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the purified enzyme is more resistant to inactivation by O2 than the enzyme in cell extract | Methanopyrus kandleri |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5026 | - |
- |
Methanopyrus kandleri |
Storage Stability | Organism |
---|---|
-20°C, under N2, 50 mM Tris/HCl buffer, pH 7, containing 1.3 M KCl, no loss of activity for several months | Methanopyrus kandleri |
-20°C, under N2, in 50 mM Tris/HCl, pH 7, containing 1.3 M KCl, stable for several months | Methanopyrus kandleri |
0°C, both under anaeobic and aerobic conditions, the enzyme also loses very little activity within 24 h | Methanopyrus kandleri |
0°C, both under anaerobic and anaerobic conditions, stable for 24 h | Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41500, SDS-PAGE | Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
thermostable up to 90°C at 0.04 M K2HPO4 | Methanopyrus kandleri |
90 | - |
60 min, 50 mM tricine/KOH, pH 8, stable | Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methanopyrus kandleri |