Any feedback?
Literature summary for 3.5.4.27 extracted from
- N5,N10-Methenyltetrahydromethanopterin cyclohydrolase from the extremely thermophilic sulfate reducing Archaeoglobus fulgidus: comparison of its properties with those of the cyclohydrolase from the extremely thermophilic Methanopyrus kandleri (1993), Arch. Microbiol., 159, 213-219.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Archaeoglobus fulgidus | 5737 | - |
| cytoplasm | - |
Methanopyrus kandleri | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K2HPO4 | 1.5 M increases the activity 1.6fold | Archaeoglobus fulgidus | |
| K2HPO4 | 1.5 M K2HPO4 required for optimal activity | Methanopyrus kandleri | |
| Na2SO4 | 1 M, can substitute for K2HPO4 in stimulation | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
2 * 39000, SDS-PAGE | Archaeoglobus fulgidus |
| 80000 | - |
non-denaturing PAGE | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
| Methanopyrus kandleri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Archaeoglobus fulgidus |
| additional information | - |
- |
Methanopyrus kandleri |
| 1765 | - |
- |
Archaeoglobus fulgidus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several weeks | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Archaeoglobus fulgidus | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no requirement of salts for thermostability | Methanopyrus kandleri |
| 90 | - |
- |
Methanopyrus kandleri |
| 90 | - |
more than 80% loss of activity after 10 min without stabilizer, completely stable for more than 50 min with addition of 1.0 M K2HPO4 | Archaeoglobus fulgidus |
| 90 | - |
1.5 M K2HPO4 is required for optimal stability | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanopyrus kandleri |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
